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长白山阔叶红松林不同深度森林土壤氧化CH4研究
作者姓名:梁战备  史奕  王琛瑞  岳进
作者单位:中国科学院沈阳应用生态研究所陆地生态过程开放实验室, 沈阳 110016
基金项目:国家自然科学基金资助项目(40171092)
摘    要:采集长白山阔叶红松林不同深度土壤,在实验室条件下测定其对高低体积分数CH4的氧化和相关菌群的数量变化。结果表明,土壤氧化CH4的能力随深度变化明显;5 - 1 5cm土层具有最大CH4氧化活性,在 40 0 μL/LCH4体积分数下,此土层干重土壤CH4最大氧化速率可达3.3nmol·h- 1·g- 1 ;25cm以下土层基本没有CH4氧化活性;因 0 - 5cm土层土壤含有高质量浓度NH+4,抑制了CH4氧化菌的活性,所以此层土壤对CH4吸收能力下降。对微生物数量统计结果显示,5 - 1 0cm土层CH4氧化菌数量最大,CH4氧化菌对林土CH4氧化贡献大,硝化菌的作用很小。

关 键 词:CH4氧化  深度特征  CH4氧化菌  
收稿时间:2002-12-30
修稿时间:2003-05-19

Methane Oxidation in Soil Profiles of Broad-Leaved/ Korean Pine Mixed Forest in Changbai Mountain
Authors:Liang Zhan-bei  Shi Yi  Wang Chen-rui  Yue Jin
Institution:Open Laboratary of Terrestrial Ecological Process Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Abstract:Samples were collected from soil in different depths in the virgin broad leavedl Korean pine mixed forest in Changbai Mountain. Laboratory study on methane oxidation of forest soil revealed the vertical profiles of methane oxidation activity. Maximum methane oxidation occurred at the horizon located at 5 to 15cm below the soil surface. When incubated with 400 μL/ L methane,it had the highest uptake rate of 3.3 nmol.h- 1g- 1.Soil at horiwn below 25 cm had negligible capacity to oxidize methane.Methane-oxidizing capacity of surface soil ( 0-5 cm) decreased because of inhibition effect of high concentration NH4 on methanotrophs. The number of methanotrophs was greatest in soil at 5-10 cm below the surface.Methanotroph contributed more to methane oxidation than nitrifer did.
Keywords:methane oxidation  vertical profile  methanotroph  
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