| 黄花菜多糖的测定方法研究 |
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| 引用本文： | 周纪东,李余动.黄花菜多糖的测定方法研究[J].南通职业大学学报,2014(3):92-95. |
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| 作者姓名： | 周纪东 李余动 |
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| 作者单位： | 1. 浙江育英职业技术学院 生物技术系,杭州,310018
2. 浙江工商大学 食品与生物工程学院,杭州,310035 |
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| 摘 要： | 研究一种准确测定黄花菜中多糖的方法。采用地衣酚-盐酸法测定黄花菜中多糖含量,以木糖作为标准对照品,检测波长650 nm。结果表明,黄花菜中多糖平均含量为15.82%,平均回收率为98.77%,RSD=2.25%（n=6）。该测定方法操作简便,重现性和稳定性较好,既适用于黄花菜多糖含量的测定,也可为其他戊糖含量高的多糖测定提供一种可行方法。
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| 关 键 词： | 黄花菜 多糖 含量测定 地衣酚-盐酸法 |
Research on Method to Measure Day-lily Polysaccharides |
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| ZHOU Ji-dong,LI Yu-dong.Research on Method to Measure Day-lily Polysaccharides[J].Journal of Nantong Vocational College,2014(3):92-95. |
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| Authors: | ZHOU Ji-dong LI Yu-dong |
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| Institution: | ZHOU Ji-dong, LI Yu-dong(1.Department of Biotechnology, Zhejiang Yuying Vocational Technology College, Hangzhou, 310018, China; 2. School of Food and Biological Engineering, Zhejiang Industry and Commerce University, Hangzhou 310035, China) |
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| Abstract: | To establish a method to measure polysaccharides we research in day-lily accurately. We use the method of Orcinol- hydrochloric acid to measure polysaccharide content in day-lily with xylose as the standard reference, and the detection wavelength is 650 nm. The results show that the average content of day-lily polysaccharides is 15.82%, the average recovery rate is 98.77%, RSD = 2.25%（n = 6）. The assay method is simple, with better reproducibility and stability, and it＇s not only applicative for the measure of polysaccharide content in day-lily, but also provides a feasible method for the measure of other polysaccharide with high pentose content. |
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| Keywords: | day-lily polysaccharides content determination orcinol-hydrochloric acid method |
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