| 黑曲霉糖化酶分离纯化与酶学性质研究 |
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| 引用本文： | 梁新红,孙俊良,唐玉,郭祖峰.黑曲霉糖化酶分离纯化与酶学性质研究[J].河南职业技术师范学院学报,2011(4):24-27. |
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| 作者姓名： | 梁新红 孙俊良 唐玉 郭祖峰 |
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| 作者单位： | 河南科技学院,河南新乡453003 |
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| 基金项目： | 河南省教育厅自然科学研究资助计划项目（2010A550004）; 河南科技学院博士基金资助项目（08001） |
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| 摘 要： | 纯化了黑曲霉Aspergillus niger FJL0801糖化酶,并对其酶学性质进行研究.粗酶液经纯化后,较粗酶液纯化了38.42倍,酶活回收率达到17.45%.酶最适作用温度为60℃;最适反应pH值为4.5;在60℃下,保温2 h后,相对酶活42%±2.8%.在pH4.5,60℃下,作用时间在60 min以内,其酶活保存89%±2.56%;其酶学性质符合淀粉糖化工业化过程中对酶的要求,该酶比较适合应用于淀粉糖化工业.
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| 关 键 词： | 糖化酶 黑曲霉 分离纯化 酶学性质 |
Purification and properties of a glucoamylase from Aspergillus niger |
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| Liang Xinhong,Sun Junliang,Tang Yu,Guo Zufeng.Purification and properties of a glucoamylase from Aspergillus niger[J].Journal of Henan Vocation-Technical Teachers College,2011(4):24-27. |
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| Authors: | Liang Xinhong Sun Junliang Tang Yu Guo Zufeng |
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| Institution: | (Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | A glucoamylase produced by Aspergillus niger FJL 0801 was purified and the properties was subsequently measured.The crude amylase was purified 38.42 times with 17.45% recovery of enzyme activity.The enzyme proerties showed that the optimum pH and temperature was 4.5 and 60℃,respectively.After a further reaction at pH 4.5 for 2h,the relative enzyme activity is 42%±2.8%;and within 60 minutes,the relative enzyme activity is 89%±2.56%.The glucoamylase is suitable for applying in starch sugar industry. |
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| Keywords: | glucoamylase Aspergillus niger purification properties |
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