Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen |
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| Authors: | Yong-liang Zhuang Xue Zhao Ba-fang Li |
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| Institution: | (1) College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China |
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| Abstract: | To optimize the hydrolysis conditions to prepare hydrolysates of jellyfish umbrella collagen with the highest hydroxyl radical
scavenging activity, collagen extracted from jellyfish umbrella was hydrolyzed with trypsin, and response surface methodology
(RSM) was applied. The optimum conditions obtained from experiments were pH 7.75, temperature (T) 48.77 °C, and enzyme-to-substrate ratio (E]/S]) 3.50%. The analysis of variance in RSM showed that pH and E]/S] were
important factors that significantly affected the process (P<0.05 and P<0.01, respectively). The hydrolysates of jellyfish umbrella collagen were fractionated by high performance liquid chromatography
(HPLC), and three fractions (HF-1>3000 Da, 1000 Da<HF-2<3000 Da, and HF-3<1000 Da) were collected. The HF-2 fraction had the
highest hydroxyl radical scavenging activity with the highest yield compared with the other two fractions. Furthermore, HF-2
also showed the strongest Cu2+-chelating ability and the best tyrosinase-inhibitory activity.
Project (No. 2008BAD94B07) supported by the Hi-Tech Research and Development Program (863) of China |
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| Keywords: | Jellyfish umbrella collagen Hydrolysis Antioxidant activity Response surface methodology (RSM) |
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