Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload |
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| Authors: | Ye-jing Zhang Mei-xiang Xiang Jiang San Gang Cheng Shi-sheng Wang |
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| Institution: | (1) Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China |
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| Abstract: | Objective: To explore the effect and mechanism of matrine (Mt.) on myocardial interstitial fibrosis induced by pressure overload.
Methods: Pressure overloaded myocardial hypertrophy was produced by banding of aorta abdominalis in 67 male Sprague-Dawley
rats weighing (200±15) g. The rats were assigned into one of the following groups: sham-operation control, operation control,
operation group treated with matrine (15 mg/(kg·d)) and treated with carvedilol (Car.) (3.6 mg/(kg·d)) group. The rats were
given drugs one day after operation. Five weeks after treatment, the left ventricular weight (LVW) was measured and the volume
of myocardial cells was detected with Hematoxylin-Eosin (H-E) stain and Masson stain was used to assess the level of fibrosis
of the myocardial matrix. Myocardial metalloproteinase activity was quantified with zymography, and survival rate was calculated.
Results: Survival rate significantly decreased (P<0.05), LVW/BW (body weight), MMP-2 (matrix metalloproteinase-2) activity (P<0.05), size of cardiomyocytes and interstitial fibrosis obviously increased in the operation group compared with sham control
group. Mt. and Car. treatment can significantly increase survival rate (P<0.05), decrease LVW/BW (P<0.05) and MMP-2 activity (P<0.05), decrease size of cardiomyocytes and interstitial fibrosis compared with operation group. But there was difference
compared with sham group. Conclusion: Matrine was shown to be able to prevent cardiac remodelling of hypertrophy cardium induced
by pressure overload including myocardial hypertrophy and fibrosis which may be associated with the decrease in MMP-2 activity
of heart. |
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| Keywords: | Matrine Carvedilol Myocardial Hypertrophy fibrosis MMP-2 |
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