| Effects of Hg and Cu on the activities of soil acid phosphatase |
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| 作者姓名： | XU Dong-mei CHEN Bo LIU Wen-li LIU Guang-shen LIU Wei-ping |
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| 作者单位： | XU Dong-mei1，CHEN Bo1，LIU Wen-li1，LIU Guang-shen1，LIU Wei-ping2 (1Institute of Environmental Science，Zhejiang University，Hangzhou 310029，China) (2Research Center of Environmental Science，Zhejiang University of Technology，Hangzhou 310032，China) |
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| 基金项目： | Project (No. 2002CB410800) supported by the National NaturalScience Foundation of China |
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| 摘 要： | Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contami- nated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.
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| 关 键 词： | 生物活度 土壤酸化 磷酸酶 生物化学 |
| 收稿时间： | 8 September 2006 |
| 修稿时间： | 2006-09-082007-02-27 |
Effects of Hg and Cu on the activities of soil acid phosphatase |
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| XU Dong-mei CHEN Bo LIU Wen-li LIU Guang-shen LIU Wei-ping.Effects of Hg and Cu on the activities of soil acid phosphatase[J].Journal of Zhejiang University Science,2007,8(7):1157-1163. |
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| Authors: | Xu Dong-mei Chen Bo Liu Wen-li Liu Guang-shen Liu Wei-ping |
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| Institution: | (1) Institute of Environmental Science, Zhejiang University, Hangzhou, 310029, China;(2) Research Center of Environmental Science, Zhejiang University of Technology, Hangzhou, 310032, China |
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| Abstract: | Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contami- nated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree. |
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| Keywords: | Acid phosphatase (ACPase) Activity Heavy metal Kinetic property Soil |
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