Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum) |
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| Authors: | Rao Abdul Qayyum Irfan Muhammad Saleem Zafar Nasir Idrees Ahmad Riazuddin Sheikh Husnain Tayyab |
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| Institution: | (1) State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, 100093 Beijing, China;(2) Office of Global Learning, Wichita State University, Wichita, KS 67260-0142, USA;(3) Geosciences Department, Florida Atlantic University, Boca Raton, FL 33431, USA;(4) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China;(5) Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001, Japan;(6) National Institute for Environmental Studies, Tsukuba 305-8506, Japan |
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| Abstract: | The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines,
QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold
increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase
in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded
up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,
with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size. |
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| Keywords: | Transformation Gossypium hirsutum Phytochrome B Overexpression Plant growth Yield |
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