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阔叶十大功劳中总黄酮的提取与含量测定
引用本文:蒋琼凤,唐克华.阔叶十大功劳中总黄酮的提取与含量测定[J].湖南科技学院学报,2005,26(11):124-126.
作者姓名:蒋琼凤  唐克华
作者单位:1. 湖南科技学院,生命科学与化学工程系,湖南,永州,425006
2. 吉首大学,资环学院,湖南,吉首,416000
摘    要:目的:从阔叶十大功劳中提取抗氧化成分总黄酮,并对其含量进行测定.方法:依据文献报道的索氏提取法、超声波法及微波法提取阔叶十大功劳叶、花中总黄酮,采用NaNO2-Al(NO3)-NaOH 显色体系的510nm分光光度法测定提取物中总黄酮.结果:微波处理法具有提取速度快、提取率高、方法重现性好的特点.以微波法提取阔叶十大功劳叶、花中总黄酮含量分别为2.182%、8.155%.

关 键 词:阔叶十大功劳      总黄酮  含量  测定
文章编号:1673-2219(2005)11-0124-03
收稿时间:09 20 2005 12:00AM
修稿时间:2005年9月20日

Study on the Flavonoids Extracting from Mahonia bealei(fort.) carr and the content Determination
JIANG Qiong-feng,TANG Ke-hua.Study on the Flavonoids Extracting from Mahonia bealei(fort.) carr and the content Determination[J].Journal of Hunan University of Science and Engineering,2005,26(11):124-126.
Authors:JIANG Qiong-feng  TANG Ke-hua
Institution:1 .Department of Life Science And Chemical Engineering,Hunan University of Science and Engineering, Yongzhou 425006,China; 2.College of Biology Resourses And Enviromental Science, Jishou University, Jishou, 416000 China
Abstract:Objective:The methods of flavonoids extracting from Mahonia bealei(fort.) can were compared and it's content were studied in this paper.Method:The flavonoids in the flowers and leaves were extracted from Mahonia bealei(fort.) carr by solex method,supersound method and microwave method reported in documents,and the content of the flavonoids was determined by NaNO_2-Al(NO_3)-NaOH Coloration system under 510nm.Results:By comparison,the microwave extraction has three advantages short time,high rate and well repetition.The content of the flavonoids in the Mahonia bealei(fort.) carr leaves and flowers by microwave extraction was 2.180% and 8.155%.
Keywords:Mahonia bealei(fort  )cart  leaves  flowers  flavonoids  contents  determination  
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