| 诸葛菜(Orychophragmus violaceus)查尔酮合成酶基因OvCHS的克隆与序列分析 |
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| 引用本文： | 倪雪莉,黄余磊,梁刚良,鲍笑笑,蒋明.诸葛菜(Orychophragmus violaceus)查尔酮合成酶基因OvCHS的克隆与序列分析[J].台州学院学报,2010,32(3). |
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| 作者姓名： | 倪雪莉 黄余磊 梁刚良 鲍笑笑 蒋明 |
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| 作者单位： | 台州学院,生命科学学院,浙江,临海,317000 |
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| 基金项目： | 生命科学学院学生科研项目，台州市科技计划项目 |
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| 摘 要： | 根据GenBank发布的基因序列,设计PCR引物,分别从诸葛菜(Orychophragmus violaceus)的花瓣基因组DNA和cDNA克隆到查尔酮合成酶基因,并定名为OvCHS,序列已上传至NCBI数据库,登陆号为EF408918。序列分析表明,OvCHS基因的基因组全长为1263 bp,具一个75 bp的内含子,编码区全长为1188 bp,编码395个氨基酸。与模式植物拟南芥(Arabidopsis thaliana)查尔酮合成酶基因AtCHS比较发现,两基因编码区有135个碱基不同,相似性为88.64%,氨基酸序列中仅16个氨基酸残基的差异,相似性达95.95%。
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| 关 键 词： | 诸葛菜 查尔酮合成酶基因 克隆 表达分析 |
Isolation and sequence analysis of a chalcone synthase gene OvCHS from Orychophragmus violaceus |
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| NI Xue-li,HUANG Yu-lei,LIANG Gangliang,BAO Xiao-xiao,JIANG Ming.Isolation and sequence analysis of a chalcone synthase gene OvCHS from Orychophragmus violaceus[J].Journal of Taizhou University,2010,32(3). |
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| Authors: | NI Xue-li HUANG Yu-lei LIANG Gangliang BAO Xiao-xiao JIANG Ming |
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| Institution: | NI Xue-li; HUANG Yu-lei; LIANG Gangliang; BAO Xiao-xiao; JIANG Ming(School of Life Science; Taizhou University; Linhai 317000; China); |
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| Abstract: | PCR primers were designed according to known gene sequences registered in GenBank.A chalcone synthase gene,designated OvCHS,was isolated from petal genomic DNA and cDNA of Orychophragmus violaceus,respectively.Gene sequence was submitted to NCBI GenBank with accession number of EF408918.Sequence analysis showed that genomic DNA of OvCHS was 1263 bp with an intron of 75 bp and a complete coding se-quence of 1188 bp in length encoding 395 amino acids.Sequence comparison with model organism Arabidopsis thalian... |
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| Keywords: | Orychophragmus violaceus chalcone synthase gene isolation sequence analysis |
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