| 拟南芥热激因子AtHsfAla在PET原核表达系统中的表达及纯化 |
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| 引用本文： | 郭丽红,戴利利,邵春燕,禹志宣.拟南芥热激因子AtHsfAla在PET原核表达系统中的表达及纯化[J].昆明师范高等专科学校学报,2011(6):75-76,84. |
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| 作者姓名： | 郭丽红 戴利利 邵春燕 禹志宣 |
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| 作者单位： | 昆明学院生命科学与技术系,云南昆明650214 |
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| 基金项目： | 国家自然科学基金资助项目（31060039）;云南省自然科学基金资助项目（2010ZC163）;昆明学院科学研究资助项目（YJL11025）;校级重点学科建设作者 |
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| 摘 要： | 以pErl30a为载体构建的表达拟南芥热激因子AtHsfAla的大肠杆菌EcoliM15（pET30a／His6一AtHsfAla）为实验材料，以IPTG诱导6XHis融合蛋白的表达并经过Ni2＋柱分离纯化AtHsfAla，再通过SDS—PAGE鉴定表达蛋白和纯化蛋白．结果显示，AtHsfAla蛋白在在PET原核表达系统中能够有效表达，并能通过亲和层析获得纯化的AtHsfAla蛋白．研究结果为揭示拟南芥热激因子AtHsfAla的作用机理奠定基础．
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| 关 键 词： | 拟南芥AtHsfAla 表达 纯化 |
Expression and Purification of Heat Shock Factor AtHsfAla of Arabidopsis Thaliana in PET Expression System |
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| GUO Li-hong,DAI Li-li,SHAO Chun-yan,YU Zhi-xuan.Expression and Purification of Heat Shock Factor AtHsfAla of Arabidopsis Thaliana in PET Expression System[J].Journal of Kunming Teachers College,2011(6):75-76,84. |
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| Authors: | GUO Li-hong DAI Li-li SHAO Chun-yan YU Zhi-xuan |
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| Institution: | ( Department of Life Science and Technology, Kunming University, Yunnan Kunming 650214, China) |
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| Abstract: | E coli M15 （ （pET30a His6-AtAtHsfAla） was used as experimental materials. Expression of AtHsfA1 a in Arabidopsis thaliana was induced with isopropyl-13-D-galactoside （IPTG）. AtHsfAla was purified by the Ni-NTA-Agarose affinity chromatography. AtHsfAla was analyzed by the SDS-PAGE eleetrophoresis. Heat shock factor HsfAla in Arabidopsis thaliana was expressed and purified. The result showed that experimental material was provided for isolating and identifying of heat shock elements （ HSE ） bound in vivo by heat shock factor HSF in Arabidopsis thaliana and founded the basis for the function mechanism of heat shock factor AtHsfAla. |
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| Keywords: | heat shock factor AtHsfAla expression purification |
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