A fluorescence based method for the quantification of surface functional groups in closed micro- and nanofluidic channels |
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| Authors: | Yu Wang Rachel D Lowe Yara X Mejia Holger Feindt Siegfried Steltenkamp Thomas P Burg |
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| Institution: | 1.Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;2.Micro Systems Technology (MST), Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany |
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| Abstract: | Surface analysis is critical for the validation of microfluidic surface modifications for biology, chemistry, and physics applications. However, until now quantitative analytical methods have mostly been focused on open surfaces. Here, we present a new fluorescence imaging method to directly measure the surface coverage of functional groups inside assembled microchannels over a wide dynamic range. A key advance of our work is the elimination of self-quenching to obtain a linear signal even with a high density of functional groups. This method is applied to image the density and monitor the stability of vapor deposited silane layers in bonded silicon/glass micro- and nanochannels. |
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