| 灯盏花素片中灯盏乙素含量的HPLC法测定 |
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| 引用本文： | 邵鑫,周洁,姚武.灯盏花素片中灯盏乙素含量的HPLC法测定[J].黄山学院学报,2012(3):53-54. |
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| 作者姓名： | 邵鑫 周洁 姚武 |
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| 作者单位： | 黄山职业技术学院;黄山学院化学化工学院 |
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| 基金项目： | 安徽省自然科学基金项目(11040606M41);安徽省教育厅自然科学研究项目(KJ2010B215);黄山学院博士人才启动项目(2009xkjq001) |
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| 摘 要： | 灯盏乙素是从中药灯盏花中提取的黄酮类有效成分。用HPLC法测定灯盏花素片中灯盏乙素的含量,采用C8反相柱,流动相为乙腈∶水∶醋酸∶2%磷酸=20∶77.5∶2.0∶0.5(V/V)的混合溶液,流速1.2ml/min,检测波长335nm。该方法在0.03-0.24μg的范围内具有良好的线性关系,平均回收率为98.05%,相对标准偏差是1.9%。此方法快速、简便,准确,可用于灯盏乙素的含量测定。
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| 关 键 词： | 灯盏花素片 灯盏乙素 高效液相色谱法 |
Determination of Scutellarin in Breviscapine Tablets by HPLC |
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| Shao Xin,Zhou Jie,Yao Wu.Determination of Scutellarin in Breviscapine Tablets by HPLC[J].Journal of Huangshan University,2012(3):53-54. |
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| Authors: | Shao Xin Zhou Jie Yao Wu |
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| Institution: | 1.Huangshan Vocational College,Huangshan245000,China; 2.School of Chemistry and Chemical Engineering,Huangshan University,Huangshan245041,China) |
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| Abstract: | Breviscapine is an active extract of flavonoids from traditional Chinese medicine Erigeron breviscapus(Vant.).An HPLC method for the determination of seutellarin in beviscapine tablets is reported.The sample is analyzed on a C8 column with a mobile phase containing acetonitrile(20%),water(77.5%),HAc(2%) and 2% H3PO4(0.5%).The flow rate and detection wavelength are 1.2 mL/min and 335 nm respectively.The calibration curve shows a good linearity within the range of 0.03-0.24μg.The average recovery is 98.05% and RSD is 1.9%.Being simple,rapid and accurate,this method can be used in the quantitative determination of scutellarin. |
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| Keywords: | breviscapine tablet scutellarin HPLC |
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