| 复合蛋白酶水解蓝圆鳆工艺条件的优化 |
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| 引用本文： | 胡阳,倪辉,杨远帆,陈申如,蔡慧农.复合蛋白酶水解蓝圆鳆工艺条件的优化[J].泉州师范学院学报,2009,27(6):98-102. |
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| 作者姓名： | 胡阳 倪辉 杨远帆 陈申如 蔡慧农 |
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| 作者单位： | 集美大学,生物工程学院,福建,厦门,361021 |
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| 摘 要： | 以水解度为优化指标，利用单因素试验考察酶解温度、pH值、时间等因素对鱼蛋白水解的影响，利用中心组合试验方法优化酶的添加量和蛋白质浓度．结果表明，复合蛋白酶水解蓝圆够的最佳工艺条件为：加酶量2700U·g^-1蛋白、蛋白质浓度1．56％、水解温度60℃、反应体系pH7．5、反应时间8h．在此条件下，水解体系的氨基酸态氮含量达1．27mg·mL^-1，水解度为50．98％，挥发性盐基氮含量为0．032mg·mL^-1。
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| 关 键 词： | 蓝圆够 复合蛋白酶 水解 优化 |
Optimization of Conditions of Hydrolysis Decapterus Maruadsi with Protamex Protease |
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| HU Yang,NI Hui,YANG Yuan-fan,CHEN Shen-ru,CAI Hui-nong.Optimization of Conditions of Hydrolysis Decapterus Maruadsi with Protamex Protease[J].Journal of Quanzhou Normal College,2009,27(6):98-102. |
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| Authors: | HU Yang NI Hui YANG Yuan-fan CHEN Shen-ru CAI Hui-nong |
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| Institution: | (School of Biotechnology,Jimei University,Fujian 361021 ,China) |
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| Abstract: | The effects of the main parameters including hydrolysis temperature, pH value, hydrolysis time were investigated with Single Factor Experiment and dosage of enzyme and protein concentration were investigated with response surface methodology （RSM）. The optimized conditions are： concentration of protamex protease is 2 700 U · g ^-1 protein,protein concentration is 1.56% ,hydrolysis temperature is 60 ℃, hydrolysis pH value is 7. 5, hydrolysis time is 8 hours. Under the conditions, amino acid nitrogen concentration attains 1.27 mg· mL-1 , hydrolysis degree attains 50.98 % and total volatile basic nitrogen reaches 0. 032 mg · mL^-1. |
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| Keywords: | Decapterus maruadsi protamex protease hydrolysis optimization |
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