| 小麦悬浮培养细胞与根癌农杆菌相互作用的亚显微生物学研究 Ⅰ.农杆菌附着于小麦细胞的扫描电镜观察 |
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| 引用本文： | 欧阳学智,许耀,李宝健.小麦悬浮培养细胞与根癌农杆菌相互作用的亚显微生物学研究 Ⅰ.农杆菌附着于小麦细胞的扫描电镜观察[J].中山大学学报论丛,1989(4). |
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| 作者姓名： | 欧阳学智 许耀 李宝健 |
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| 基金项目： | 国家“七五”重点攻关项目 |
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| 摘 要： | 本文首次用扫描电镜研究了农杆菌与小麦悬浮培养细胞之间的相互作用。在含复合诱导成份的特殊共培养条件下,农杆菌能识别和大量附着到小麦悬浮细胞表面,并形成大量的纤维丝。还揭示了共培养过程中,农杆菌与小麦细胞相互作用的空间关系以及植物细胞壁、细胞状态与农杆菌附着及转化的关系。
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| 关 键 词： | 小麦悬浮细胞 农杆菌附着 纤维丝 共培养 扫描电镜 |
Ultrastructural studies on the Interaction between Cultured Wheat Cells and Agrobacterium tumefaciens |
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| Ouyang Xuezhi Xu Yao Li Baojian.Ultrastructural studies on the Interaction between Cultured Wheat Cells and Agrobacterium tumefaciens[J].Supplement to the Journal of Sun Yatsen University,1989(4). |
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| Authors: | Ouyang Xuezhi Xu Yao Li Baojian |
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| Institution: | Ouyang Xuezhi Xu Yao Li Baojian |
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| Abstract: | The interaction of A. tumefaciens with suspension cultured cells of Triticum aestivum was investigated using scanning electron microscope for the first time. In a special medium containing complex-induced components, Agrobacteria were capable of recognizing and attaching to the surface of wheat cells, thus forming large amount of cellulose fibrils. The special relationship between agrobacteria and the surface structure of wheat cells and the relationship between Agrobacterium attachment and plant cell state and further trasformation were also discussed in this report. |
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| Keywords: | wheat suspension culture cell Agrbacterium turnefaciens attachfment cellulose fibril co-cultivation scanning electron microscope |
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