| 草莓提取物对脂肪酸合酶及脂肪细胞的抑制作用 |
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| 引用本文： | 刘晓鑫,田维熙,马晓丰.草莓提取物对脂肪酸合酶及脂肪细胞的抑制作用[J].中国科学院研究生院学报,2010,27(6):768-777. |
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| 作者姓名： | 刘晓鑫 田维熙 马晓丰 |
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| 作者单位： | 中国科学院研究生院生命科学学院,北京,100049 |
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| 基金项目： | 国家自然科学基金(30572252,30670455)、中国科学院研究生院院长基金(O95101PY00)和王宽诚教育基金会项目资助 |
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| 摘 要： | 脂肪酸合酶(FAS)是治疗肥胖症的潜在靶点.实验测定表明,草莓果肉渣及草莓叶的乙醇提取物再经乙酸乙酯萃取的有效组分对FAS具有强抑制作用,且分别对FAS的底物丙二酸单酰辅酶A、乙酰辅酶A呈非竞争性和竞争性抑制.经HPLC-MS分析,槲皮素和鞣花酸可能是草莓有效组分中起主要作用的物质.草莓叶及果肉渣乙酸乙酯提取组分均可有效抑制3T3-L1前脂肪细胞中脂滴积累,且呈剂量依赖性.实验结果对于研究草莓在防治肥胖症的应用上可能具有重要价值.
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| 关 键 词： | 脂肪酸合酶 草莓 抑制剂 抑制动力学 减肥 |
| 收稿时间： | 2010-04-20 |
Inhibitory effects of strawberry extracts on animal fatty acid synthase |
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| LIU Xiao-Xin,TIAN Wei-Xi,MA Xiao-Feng.Inhibitory effects of strawberry extracts on animal fatty acid synthase[J].Journal of the Graduate School of the Chinese Academy of Sciences,2010,27(6):768-777. |
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| Authors: | LIU Xiao-Xin TIAN Wei-Xi MA Xiao-Feng |
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| Institution: | College of Life Sciences, Graduate University, Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Fatty acid synthase (FAS) has been considered as a potential new therapeutic target for obesity. In the present study we show that the components of both strawberry ( Fragaria ananassa Duch) fruit dreg and strawberry leaves that extracted with 50% ethanol and partitioned by ethyl acetate are potent FAS inhibitors. The ethyl acetate fractions of strawberry fruit dregs (SF-AE) show noncompetitive inhibition with the substrate Mal-CoA, while the ethyl acetate fractions of strawberry leaves (SL-AE) show competitive inhibition with Ac-CoA. Based on the HPLC-MS analysis, we conclude that ellagic acid and quercetin are the efficient components. SF-AE and SL-AE could reduce lipids accumulation in 3T3-L1 preadipocyte with dose-dependence. These results may be of great value for application of strawberry extracts to obesity prevention and cure. |
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| Keywords: | fatty acid synthase strawberry inhibitor kinetic assay anti-obesity |
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