Anomalous Activity Measurements of Creatine (Phospho) Kinase,CK-MB Isoenzyme in Indian Patients in the Diagnosis of Acute Coronary Syndrome |
| |
| Authors: | Jude Joseph Fleming Harish P Janardhan Arun Jose R Selvakumar |
| |
| Institution: | (1) Department of Clinical Biochemistry, Christian Medical College, Vellore, 632004, Tamil Nadu, India;(2) Department of Biochemistry, Christian Medical College, Vellore, 632002, Tamil Nadu, India |
| |
| Abstract: | In the present study, the cause of suspected false-positive (anomalous) values for CK-MB activity, in Indian patients investigated
for ACS. Total serum CK and CK-MB activity, serum Troponin I were measured and CK-MB as a percentage of the total CK activity
(%CK-MB) calculated. CK-MB was also estimated using densitometry and CK-MB mass assay. Anomalous specimens were tested for
the presence of CK isoenzymes. In 22 healthy subjects, 11 male and female, the %CK-MB ranged from 3.6 to 30.2. In 11 male
patients, with proven ACS, the %CK-MB was from 4.0 to 17.5. The cut off for anomalous CK-MB activity values was set as >33.0%.
In 35 patients with anomalies, total CK values ranged from 39 to 231 U/L, CK-MB from 30 to 161 U/L. Investigation of CK isoenzymes,
showed 10 patients had a CK-BB band, 14 an intermediate band between CK-MM and CK-MB (macro-CK type 1), 7 had a cathodal band
(macro-CK type 2), and 3 had a band intermediate between CK-MB and CK-BB. This later band does not seem to have been previously
reported. Against the CK-MB mass assay, the activity assay showed no correlation, in 43 patients (19 M, 24 F), Pearson coefficient
(R2) was 0.006. The CK-MB immunoinhibition assay is better described as measuring “non-CK-MM activity.” A %CK-MB activity >6%
as a marker of ACS is not valid in our patient population. Laboratories should not use only CK-MB activity as a biochemical
marker of ACS. |
| |
| Keywords: | CK-MB isoenzymes Macro-creatine kinase Anomalies Biochemical markers Acute coronary syndrome |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
