In vitro study on ribozyme against hepatitis C virus |
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| Authors: | Chen Zhi Liu Yong Liu Ke-zhou R H Dennin Dou Jun U Reinhard |
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| Institution: | (1) Institute of Infectious Diseases, Medical College, Zhejiang University, 310003 Hangzhou, China;(2) Institute of Microbiology, Lubeck Medical University, Germany |
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| Abstract: | Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and
inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region,
were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued
and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine
the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%,
respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion:
Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better
effect when used together. |
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| Keywords: | ribozyme inhibition hepatitis C virus MT-2 cells |
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