Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles |
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| Authors: | Jaideep S Dudani Daniel R Gossett Henry T K Tse Robert J Lamm Rajan P Kulkarni Dino Di Carlo |
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| Institution: | 1Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, USA;2California NanoSystems Institute, Los Angeles, California 90095, USA;3Jonsson Comprehensive Cancer Center, Los Angeles, California 90095, USA;4Division of Dermatology, David Geffen School of Medicine, Los Angeles, California 90095, USA |
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| Abstract: | Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches to improved isolation require specialized equipment and extensive sample preparation time. Therefore, isolation and detection methods of exosomes will benefit biological and clinical studies. Here, we report a microfluidic platform for inline exosome isolation and fluorescent detection using inertial manipulation of antibody-coated exosome capture beads from biological fluids. |
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