| 印度芥菜重金属ATP酶基因的分离与表达 |
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| 作者姓名： | 张玉秀 张媛雅 柴团耀 |
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| 作者单位： | 1. 中国矿业大学化学与环境工程学院, 北京 100083;
2. 中国科学院研究生院生命科学学院, 北京 100049 |
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| 基金项目： | 国家转基因生物新品种培育重大专项 (2009ZX08009-130B)和中央高校基本科研业务费专项资金(2010YH05)资助 |
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| 摘 要： | 利用同源序列克隆技术在印度芥菜幼苗中分离出2个重金属ATP酶cDNA片段BjHMA 3 和BjHMA 4 ;实时荧光定量PCR表明BjHMA 3和BjHMA4 在所有的组织器官中均有表达,其中在根中表达量最高,叶片中表达量最少;重金属Zn或Cd胁迫均能增强二者在叶片中的表达,表明BjHMA不仅参与植物的生长发育过程,而且在重金属稳态和耐性中具有重要作用.
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| 关 键 词： | 印度芥菜 重金属ATP酶 基因表达 |
| 收稿时间： | 2010-06-01 |
| 修稿时间： | 2010-09-14 |
Isolation and expression of heavy metal ATPase in Brassica juncea L. |
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| Authors: | ZHANG Yu-Xiu ZHANG Yuan-Ya CHAI Tuan-Yao |
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| Institution: | 1. School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing 100083, China;
2. College of Life Science, Graduate University, Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Brassica juncea L. is a Zn/Cd tolerance plant, and the gene expression of heavy metal ATPase (HMA) has not been reported. In the present work, two cDNA fragments of BjHMA, BjHMA 3 and BjHMA 4 , were isolated from B. juncea seedling. Real-time quantitative PCR analysis revealed that both BjHMA 3 and BjHMA 4 were constitutively expressed in all tissue and preferentially in root. The mRNA profile of BjHMA 3 or BjHMA 4 was the lowest in leaf while the expressions were strongly enhanced by Zn or Cd stress, indicating that BjHMA played important roles in growth and in heavy metal homeostasis and tolerance in plant. |
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| Keywords: | Brassica juncea L heavy metal ATPase gene expression |
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