| HPLC法同时测定氧化槐果碱、槐定碱和苦参碱 |
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| 作者姓名： | 吴志娇 赵红 薛英 胡新明 毛一卿 谭焕然 |
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| 作者单位： | 1. 中国科学院研究生院, 北京 100049;
2. 北京大学, 北京 100191 |
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| 基金项目： | 国家科技支撑计划(2009BAK61B01)、国家重点基础研究发展计划(2007CB935900)和中国科学院长春应用化学研究所电分析化学开放研究实验室基金(SKLEAC2010005)资助 |
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| 摘 要： | 建立了同时测定氧化槐果碱、槐定碱和苦参碱的HPLC(高效液相色谱)方法.用VP-ODS(维普C18柱)150mm × 4.6 mm, 5 μm 色谱柱,柱温40 ℃,流动相为0.01mol/L pH 8.5磷酸盐缓冲溶液-甲醇(40 ∶60),流速 1.0 mL/min,紫外检测器波长215 nm.结果表明,3种生物碱在确定的色谱条件下得到很好的分离,且在所选质量浓度范围20~200 mg/L 内线性关系良好,回收率范围97.2%~102%.
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| 关 键 词： | 氧化槐果碱 槐定碱 苦参碱 高效液相色谱 |
| 收稿时间： | 2010-05-17 |
| 修稿时间： | 2010-09-06 |
Simultaneous determination of oxysophocarpine,sophoridine, and matrine by HPLC |
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| Authors: | WU Zhi-Jiao ZHAO Hong XUE Ying HU Xin-Ming MAO Yi-Qing TAN Huan-Ran |
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| Institution: | 1. Graduate University, Chinese Academy of Sciences, Beijing 100049, China;
2. Peking University, Beijing 100191, China |
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| Abstract: | An HPLC method for simultaneous determination of oxysophocarpine, sophoridine, and matrine was proposed. VP-ODS column (150 mm × 4.6 mm, 5 μm) was used in the experiment. Column temperature was 40 ℃, mobile phase was 0.01 mol/L phosphate buffer solution (pH 8.5)-methanol (40 ∶60), UV detection wavelength was 215nm, and flow rate was 1.0 mL/min. The results indicated that the three kinds of alkaloid were separated effectively and showed good linearity within the selected concentration range. |
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| Keywords: | oxysophocarpine sophoridine matrine HPLC |
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