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| 反射仪—K+试纸法快速测定烟草含K+水平的方法研究 | |
| 引用本文： | 张晓海,廖德智,黄成江司马永康.反射仪—K+试纸法快速测定烟草含K+水平的方法研究[J].中国科学院研究生院学报,2006,23(1):4-6. |
| 作者姓名： | 张晓海 廖德智 黄成江司马永康 |
| 作者单位： | (中国科学院武汉植物园 武汉 430074) (云南省林业科学院 昆明 650204) |
| 摘 要： | 比较了反射仪—K+试纸法(K+ts-fl)、原子吸收光谱法、ICP–aeS法测定烟株叶脉汁液中的K+含量的三种方法，确定反射仪—K+试纸法测定烟株钾含量的可行性及其最佳测定范围；在烤烟不同生育期，应用反射仪—K+试纸法对烟株叶片不同部位含K+水平的测定，确定其最佳测定时期及部位。研究结果表明，反射仪—K+试纸法与原子吸收光谱法、ICP–aeS法测定结果差异不显著，应用反射仪—K+试纸法进行烟株钾素快速诊断可行，且具有时间短，简单快速，易操作等优点。反射仪测定K+的稳定线性范围是0.30g/L~0.9g/L；在不同时期对烟株不同部位测定研究表明，最佳诊断部位为烟株第二平展叶叶脉基部2cm段。 |
| 关 键 词： | 烤烟 反射仪—K+试纸法 K+含量 测定方法 |
| 收稿时间： | 2004-08-06 |
| 修稿时间： | 2005-11-22 |
A karyomorphological study of 40 species in 11 genera of the Magnoliaceae from China |
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| MENG Ai-Ping,WANG Heng-Chang,LI Jian-Qiang,SIMA Yong-Kang.A karyomorphological study of 40 species in 11 genera of the Magnoliaceae from China[J].Journal of the Graduate School of the Chinese Academy of Sciences,2006,23(1):4-6. | |
| Authors: | MENG Ai-Ping WANG Heng-Chang LI Jian-Qiang SIMA Yong-Kang |
| Institution: | (Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan 430074, China) (Yunnan Academy of Forestry, Kunming 650204, China) |
| Abstract: | In this paper, 40 species in 11 genera of the Magnoliaceae from China were karyomorphologically investigated for a better understanding of the intergeneric relationships in the family and the systematic positions of some species, particularly those in the genus Manglietia. The 20 Manglietia species studied are all diploid with 2n=38, indicating that in this genus the speciation may have taken place predominantly at the diploid level and that minor structural changes of chromosomes may have resulted in distinct morphological divergence. In the genus Magnolia, cytotypes with various ploidy levels from 2x to 6x have been found, which may be, at least to some degree, associated with the wide distributional range and complex gross-morphological variation of the genus. Our results indicate that Manglietia should be an independent genus from Magnolia and do not support the treatment of merging them as a genus. All the Michelia species already with chromosome numbers are diploid with 2n=38, while most of the species in Magnolia subgen. Yulania are polyploid, suggesting that Michelia is not closely allied with Yulania and that the two groups should not be merged as a genus. All Parakmeria members are polyploid. It is noteworthy that cytological evidence is generally less informative in clarifying the intergeneric relationships of the Magnoliaceae than the interspecific relationships within a given genus of the family because reticulate evolution may have extensively occurred in this family. Systematic positions of some species in Manglietia are discussed based on evidence from cytology, morphology and geographical distribution. |
| Keywords: | Magnoliaceae karyotype karyomorphology chromosome number China |
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