| 生淀粉糖化酶高产菌株诱变选育 |
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| 引用本文： | 孙子羽,迟乃玉,李兵,王宇,张庆芳.生淀粉糖化酶高产菌株诱变选育[J].大连大学学报,2010,31(6):75-77. |
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| 作者姓名： | 孙子羽 迟乃玉 李兵 王宇 张庆芳 |
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| 作者单位： | [1]大连大学生物工程学院,辽宁大连116622 [2]大连轻工业大学生物与食品工程学院,辽宁大连116034 |
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| 摘 要： | 以细菌Aeromonas.sp.CNY01为出发菌株,通过紫外（UV）和硫酸二乙酯（DES）诱变,获得产酶较高的菌株CNY01-27-13,酶活达到98.42 U/mL,比原始菌株酶活提高了110.52（,菌株经过9次传代,酶活较稳定。
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| 关 键 词： | 生淀粉糖化酶 紫外诱变 DES诱变 突变株 |
Mutating of raw starch-digesting glucoamylase strain |
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| SUN Zi-Yu,CHI Nai-Yu,LI Bing,WANG Yu,ZHANG Qing-Fang.Mutating of raw starch-digesting glucoamylase strain[J].Journal of Dalian University,2010,31(6):75-77. |
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| Authors: | SUN Zi-Yu CHI Nai-Yu LI Bing WANG Yu ZHANG Qing-Fang |
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| Institution: | 1.Bioengineering College,Dalian University,Dalian,11622,China;2.School of Biological and Food Engineering,Dalian Polytechnic University,Dalian,116034,China) |
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| Abstract: | A mutant strain CNY01-27-13 with high yied of raw starch-digesting glucoamylase was isolated from protoplast of Aeromonas.sp.CNY01 treated by UV and DES.The raw starch-digesting glucoamylase activity of the strain raised to 98.42 U/mL,which was 110.52 % higher than the enzyme activity of protoplast strain.The enzyme activity of the mutant strain was very steady cultured by nine times. |
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| Keywords: | raw starch-digesting glucoamylase UV mutagenesis DES mutagenesis mutant |
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