| 紫外线诱变庆大霉素产生菌育种与发酵条件的研究 |
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| 引用本文： | 龙石红,邓斌.紫外线诱变庆大霉素产生菌育种与发酵条件的研究[J].湘南学院学报,2002,23(5):120-122. |
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| 作者姓名： | 龙石红 邓斌 |
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| 作者单位： | 1. 永州职业技术学院,生物工程系,湖南,永州,425000
2. 郴州师范高等专科学校,化学系,湖南,郴州,423000 |
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| 摘 要： | 以紫外线为诱变剂,用庆大霉素产生菌JY1-12为出发菌株,选育出性能优良的菌株JY3-5.经正交试验确定其最佳发酵培养基,研究了最佳发酵温度和初始pH值,使其摇瓶效价提高35.1%;30m3灌放大试验,比出发菌株效价提高19.2%.
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| 关 键 词： | 紫外线诱变 庆大霉素 育种 发酵 |
| 文章编号： | 1008-2042(2002)05-0120-03 |
| 修稿时间： | 2002年7月4日 |
Selection of Micromonospora Purpurea by UV-Mutagenesis and Studies on Fermentation Conditions |
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| Abstract: | The start strain of JY1 12 was treated with UV rayirradiation to obtain a strain of micmmonospora purpurea JY3 5 with high yield gentamich. The culture medium was optimizied for the strain JY3 5 by L9(34)test, and the effective factors on fermentation was also studied. The fermentation potency of strain JY3 5 in shaker. the increased by 34.3%, and in 30m3 fermentor its increase was 18.6% as compared with JY1 12. |
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| Keywords: | UV mutagenesis gentamicin selection fermentation |
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