| Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination |
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| 作者姓名： | 吴小锋 曹翠平 |
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| 作者单位： | Lab of Gene Engineering，College of Animal Sciences，Zhejiang University，Lab of Gene Engineering，College of Animal Sciences，Zhejiang University Hangzhou 310029，China，Hangzhou 310029，China |
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| 基金项目： | Project supported by the ScientificResearch Foundation for
Returned Overseas Chinese Scholars,Education Ministry of Chinaand the Natural Science Foundation of Zhejiang Province (No.301306), China |
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| 摘 要： | INTRODUCTION Transgenic technology developed over the 20years, has become a common tool widely applied tolivestock species because it is technically quitesimple, requiring only the injection of ‘naked’DNA into the nucleus of a fertilized eggs (Hammeret al., 1985; Suraokar and Bradley, 2000). Howeverthis technique has limitations in that the injectedDNA integrates randomly into the genome and thuscould not be expressed in the desired tissue or at theappropriate level. More impor…
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Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination |
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| WU Xiao-feng,CAO Cui-ping.Targeting of human aFGF gene into silkworm,Bombyx mori L. through homologous recombination[J].Journal of Zhejiang University Science,2004(6). |
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| Authors: | WU Xiao-feng CAO Cui-ping |
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| Abstract: | The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through ho- mologous recombination. |
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| Keywords: | Gene targeting Recombinant vector Bombyx mori L |
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