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Oscillating Cellulase Adsorption and Enhanced Lignocellulose Hydrolysis upon Ultrasound Treatment
Authors:Rongxin  Su  Renjun  Yang  Yang Jifeng  Ruoyu  Du  Renliang  Huang  Wei  Qi  Zhimin  He
Institution:1.State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology,Tianjin University,Tianjin,China;2.Collaborative Innovation Center of Chemical Science and Engineering (Tianjin),Tianjin,China;3.Chongqing Key Laboratory of Catalysis and Functional Organic Molecules, College of Environment and Resource,Chongqing Technology and Business University,Chongqing,China;4.School of Environmental Science and Engineering,Tianjin University,Tianjin,China
Abstract:We investigated the effects of ultrasound treatment on cellulase adsorption and lignocellulose hydrolysis. The activity of cellulase remained constant upon low-power ultrasound treatment (<120 W) and decreased using high-power ultrasound (>280 W). Oscillating cellulase adsorption occurred upon ultrasound treatment with any intensity. The maxima for desorption and adsorption were 41.9 and 83.1%, respectively, during 1 h of 90 W ultrasound treatment at 50 °C. A comparison between the short-time with long-time ultrasound experiments indicated that ultrasound treatment tended to desorb cellulase from substrate. However, ultrasound treatment also led to further surface erosion of biomass, which increased cellulase accessibility. These joint actions of ultrasound treatment induced the oscillating adsorption of cellulase. The increase in cellulase accessibility caused by ultrasound treatment led to a significant enhancement in lignocellulose hydrolysis.
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