| 鲁米诺流动注射化学发光法测定呋喃西林的含量 |
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| 引用本文： | 叶瑞洪,林谦,吴琳,卢贵文.鲁米诺流动注射化学发光法测定呋喃西林的含量[J].福建师大福清分校学报,2013(5):47-52. |
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| 作者姓名： | 叶瑞洪 林谦 吴琳 卢贵文 |
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| 作者单位： | 福建师范大学福清分校生物与化学工程系,福建福清350300 |
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| 基金项目： | 福建省教育厅A类科技项目，食品安全分析与检测教育部重点实验室（福州大学）开放课题项目，福建省大学生创新实验项目（20421134）资助。 |
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| 摘 要： | 基于过氧化氢与鲁米诺化学发光反应，并应用MPI-F型流动注射化学发光仪分析测定呋喃西林含量的方法。该方法的最佳测定条件：H2O20.10mol/l，NaOH0.10mol/l，鲁米诺5.0×10-6mol/l，负高压为800V，蠕动泵主泵运行10s后副泵运行30s，接着主泵继续转动10s，副泵30s，如此循环，上述化学发光强度因呋喃西林的存在而显著减弱，且其强度的减弱值（ΔI）与呋喃西林浓度在0.0500-2.00μg/ml之间呈线性关系，方法的检出限为0.0100μg/ml。对0.100μg/ml的呋喃西林进行11次平行测定，其相对标准偏差为6.50%。该法已成功测定了三种不同浓度呋喃西林精粉的含量，其相对标准偏差分别为2.01%、1.25%、3.50%，平均回收率在98.8%-100.5%之间。
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| 关 键 词： | 流动注射化学发光 鲁米诺-过氧化氢 呋喃西林 |
Determination of the Content of Furacilin by Luminol Flow Injection Chemiluminescence |
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| YE Ruihong,LIN Qian,WU Lin,LU Guiwen.Determination of the Content of Furacilin by Luminol Flow Injection Chemiluminescence[J].Journal of Fuqing Branch of Fujian Normal University,2013(5):47-52. |
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| Authors: | YE Ruihong LIN Qian WU Lin LU Guiwen |
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| Institution: | (Fuqing Branch of Fujian Normal University, Fuqing, Fujian 350300) |
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| Abstract: | the experiment in this paper is based on the chemiluminescence emission produced upon mixing a hydrogen peroxide solution with an alkaline luminol solution and a MPI-F flow-injection chemiluminescence instrument was employed as well. The optimum condition for this method is described as below：H2O20.1mol/1, NaOH0.1mol/1, luminol 5.0x10-6mol/1, negative high voltage 800V, main peristaltic pump runs for 10s and entailed by sub-peristaltic pump for 30s and this cycle is repeated;the intensity of chemiluminescence was apparently weakened due to the existence of Furacilin, and the weakening value （△I） has a linear relation with the content of Furacilin at 0.0500-2.00μg/mL, the determination limit of this method is 0.0100μg/mL. 11 parallel measurements were carried out upon 0.0100μg/mL Furacilin, the relative standard deviation is 6.50%. This method has successfully measured the content of Furacilin of three different condensations, the relative deviation were 2.01%, 1.25% and 3.50%respectively;the average recycling rates are between 98.8%and 100.5%. |
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| Keywords: | flow injection chemiluminescence luminol furacilin |
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