A microfluidic chip for highly efficient cell capturing and pairing |
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| Authors: | Cui Shaoyan Liu Yaoping Wang Wei Sun Yan Fan Yubo |
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| Institution: | 1School of Biological Science and Medical Engineering, Beihang University; Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing 100191, China;2Institute of Microelectronics, Peking University; National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Beijing 100871, China |
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| Abstract: | This paper examined the feasibility of a microfluidics chip for cell capturing and pairing with a high efficiency. The chip was fabricated by the polydimethylsiloxane-based soft-lithography technique and contained two suction duct arrays set in parallel on both sides of a main microchannel. Cells were captured and paired by activating two sets of suction ducts one by one with the help of syringe pumps along with switching the cell suspensions inside the main microchannel correspondingly. The effects of suction flow rate and the dimensions of suction channels on the cell capturing and pairing efficiency were characterized. The present chip was capable of creating 1024 pairs of two different cell populations in parallel. The preliminary experimental results showed that the cell capturing efficiency was 100% and the pairing one was 88% with an optimal suction rate of 5 μl/min in the chip in the 2 μm-sized suction duct chip. The cell viability after capture inside the microfluidic device was 90.0?±?5.3%. With this cell capturing and pairing chip, interaction between cells in a single pair mode can be studied. The ability to create cell pairs has a number of biological applications for cell fusion, cell-cell interaction studies, and cell toxicity screening. |
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