| Characterization of cellulose acetate micropore membrane immobilized acylase Ⅰ |
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| 引用本文： | 郭永胜,王杰,宋锡谨.Characterization of cellulose acetate micropore membrane immobilized acylase Ⅰ[J].浙江大学学报(B卷英文版),2004(12). |
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| 作者姓名： | 郭永胜 王杰 宋锡谨 |
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| 作者单位： | Department of Chemistry，Zhejiang University，Hangzhou 310027，China，Department of Chemistry，Zhejiang University，Hangzhou 310027，China，Department of Chemistry，Zhejiang University，Hangzhou 310027，China |
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| 基金项目： | Project(No.20276067)supportedbytheNationalNaturalScienceFoundationofChina |
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| 摘 要： | This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 ℃, which is higher than that of free acylase I (60 ℃). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.
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