| 破伤风类毒素单克隆抗体的制备及初步鉴定 |
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| 引用本文： | 张秀昌,刘芳,崔萍,孙黎,罗强,贾天军.破伤风类毒素单克隆抗体的制备及初步鉴定[J].河北北方学院学报(医学版),2007,24(4):16-17. |
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| 作者姓名： | 张秀昌 刘芳 崔萍 孙黎 罗强 贾天军 |
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| 作者单位： | 1. 河北北方学院医学技术学院,河北,张家口,075000
2. 河北北方学院实验中心,河北,张家口,075000 |
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| 摘 要： | 目的：制备并鉴定破伤风类毒素单克隆抗体杂交瘤细胞株,为破伤风的快速检测及致病机制的研究提供实验材料。方法：用破伤风类毒素做抗原免疫BALB/C小鼠,取其脾细胞与小鼠骨髓瘤细胞融合,获得分泌高滴度针对破伤风类毒素的杂交瘤细胞株,测定单抗免疫球蛋白亚类及单抗效价,用间接ELISA和Western-blot检测单克隆细胞株的特异性。结果：通过细胞融合和克隆化,筛选出3株持续分泌抗破伤风类毒素单克隆抗体的杂交瘤细胞株1E12、1E11、1G10。间接ELISA和Western-blot检测结果表明1E12、1E11、1G10可以和破伤风类毒素发生特异性反应。结论：成功制备了抗破伤风类毒素单克隆抗体,为制备免疫诊断试剂盒和抗体药物的开发奠定了基础。
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| 关 键 词： | 破伤风类毒素 杂交瘤 单克隆抗体 |
| 文章编号： | 1673-1484（2007）04-0016-02 |
Preparation and Identification of Monoclonal Antibodies Against Tetanic Toxoid |
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| ZHANG Xiu-chang,LIU Fang,CUI Ping,et al.Preparation and Identification of Monoclonal Antibodies Against Tetanic Toxoid[J].Journal of Hebei North University:Medical Edition,2007,24(4):16-17. |
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| Authors: | ZHANG Xiu-chang LIU Fang CUI Ping |
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| Institution: | Medicine Technology College, Hebei North University, Zhangjiakou, 075000, Hebei, China |
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| Abstract: | Objective: To establish and prepare hybridoma cell line secreting monoclonal antibody(McAb) against tetanic toxoid,to develop a rapid assay for Tetanus and investigate the pathogenesis of the virus.Methods: BALB/c mouse was immunized by purified tetanic toxoid.Hybridoma cell lines secreting monoclonal antibodies(McAbs) against tetanic toxoid were screened after the fusion of mouse splenic cells with SP2/0 cells.The Ig subtypes and titer of the McAbs were assayed with EL ISA and Western-blot assay.Results: After cell fusion and subcloning,three hybridoma cell lines secreting monoclonal antibodies specifically against tetanic toxoid were obtained,and respectively named as 1E12,1E11 and 1G10.Indirect EL ISA and Western-blot assay showed that the McAbs 1E12,1E11 and 1G10 could specifically recognize tetanic toxoid.Conclusion: The specific anti-tetanic toxoid McAbs are developed and these McAbs may be useful in the development of detection assay and basic research of the pathogenesis of Tetanus. |
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| Keywords: | Tetanic toxoid Hybridoma cell line Monoclonal natibodies |
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