Multiple matrix metalloproteinases in type II collagen induced arthritis |
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| Authors: | S Sandya M A Achan P R Sudhakaran |
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| Institution: | (1) Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, 695 581, India;(2) Department of Orthopedics, Medical College & Hospital, Thiruvananthapuram, 695 011, India;(3) Department of Biochemistry University of Kerala, Kariavattom Thiruvananthapuram, 695 581, India |
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| Abstract: | To study the relative contribution of various matrix degrading enzymes in the pathogenesis of arthritis, changes in the levels
of various matrix metalloprtoteinases (MMPs) during the progression of collagen induced arthritis was studied in experimental
animals. Arthritis was induced in male wistar rats by injecting an emulsion containing collagen type II and Freund’s complete
adjuvant. The duration of the experiment was 35 days. Synovial effusate was collected at regular intervals after induction.
At the end of the experiment serum and cartilage were collected and analysed. Synovial fluid of osteoarthritic patients was
also analyzed. Levels of MMP-2, MMP-3, MMP-9 and MT1-MMP were found to be high in synovial effusate and cartilage of experimental
animals. In synovial effusate of arthritic animals the expression of MMP-3 was found to be high during the early stages while
increase in MMP-2 and MMP-9 occurred at later stages. Synovial fluid of osteoarthritic patients also showed elevated levels
of MMP-2, MMP-3 and MMP-9. Our results indicated that sequential action of MMPs such as MMP-3, MMP-2 and MMP-9 can cause degradation
of articular cartilage extracellular matrix. |
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| Keywords: | Matrix metalloproteinases Osteoarthritis Synovial effusate Synovial fluid |
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