Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes |
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| Authors: | Lu Xiao-feng He Guo-qing Yu Hai-ning Ma Qi Shen Sheng-rong Das Undurti N |
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| Institution: | (1) Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, 310029 Hangzhou, P R, China;(2) College of Pharmaceutical Sciences, Zhejiang University of Technology, 310032 Hangzhou, P R, China;(3) UND Life Sciences, 13800 Fairhill Road, #321, 44120 Shaker Heights, OH, USA;(4) Jawaharlal Nehru Technological University, 533 003 Kakinada, India;(5) Krishna Institute of Medical Sciences, 500 003 Secunderabad, India |
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| Abstract: | Polyunsaturated fatty acids (PUFAs) possess anti-cancer action both in vitro and in vivo. In the present study, we detected
cell viability with methyl thiazolyl tetrazolium (MTT) assay and cell membrane permeability with propidium iodide (PI) fluorescence
dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured
by gas chromatography/mass spectroscopy (GC/MS), and the relationship between fatty acid composition and cell viability was
studied. We observed that n-6 PUFA linoleic acid (LA) inhibited tumor cell growth at high concentrations (≥300 μmol/L), while low concentrations (100–200
μmol/L) seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty
acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 μmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic
action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed
that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to cell differentiation status. |
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| Keywords: | Anti-cancer Fatty acids composition Linoleic acid In vitro |
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