Binding and degradation of native and acetylated low density lipoproteins by monocyte derived macrophages of normal and hypercholesterolemic rabbits |
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| Authors: | Sabari Das Snehlata L M Srivastava |
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| Institution: | (1) Department of Nuclear Medicine, All India Institute of Medical Sciences, New Delhi;(2) Department of Biochemistry, All India Institute of Medical Sciences, 110 029 New Delhi |
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| Abstract: | Low density lipoprotein (LDL) was isolated from normal rabbits and was modified with acetic anhydride. Blood monocyte derived
macrophages from normal and hypercholesterolemic (HC) rabbits were cultured, and on the 8th day the cells were incubated with
native and modified LDL to study their binding and degradation. Macrophages from both normal and hypercholesterolemic rabbits
express a limited number of receptors for normal plasma LDL whereas they exhibit a large number of receptors for acetyl LDL.
There was no significant difference between binding and degradation of acetyl LDL by normal or hypercholesterolemic cells.
However, binding and degradation of native LDL by monocytes of hypercholesterolemic cells were significantly less (p<0.0001)
in comparison to binding and degradation of native LDL by normal cells indicating that there is a feedback inhibitory pathway
in the cell that inhibits the synthesis of LDL receptors in the presence of excess LDL. |
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| Keywords: | Monocyte Macrophage Hypercholesterolemia Low Density Lipoprotein |
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