| 伤寒免疫学诊断方法的研究现状 |
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| 引用本文： | 鲍行豪.伤寒免疫学诊断方法的研究现状[J].科技通报,1991,7(6):354-359. |
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| 作者姓名： | 鲍行豪 |
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| 作者单位： | 浙江省卫生防疫站 杭州310009 |
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| 摘 要： | 对近年伤寒免疫学诊断方法结合作者的研究工作进行了评价。伤寒免疫学诊断可分检测早期病人体液中抗原及测定患者血清中抗体两个方面。检测抗原以SPA-CoA与ELISA(包括Dot-ELISA)较为敏感和特异,而DRLAT则有简便的优点。测定抗体以PHA最为简便与快速。使用LPS-PHA可作伤寒的早期快速诊断,而Vi-PHA可作伤寒病后带菌者的初筛。ELISA有较PHA更高的敏感性,其中LPS-IgM-ELISA亦可作伤寒病人的早期诊断,而LPS-IgG-ELISA可用于该病的追溯诊断和血清流行病学调查。Vi-ELISA还可作伤寒病后带菌者初筛。不同条件实验室可根据取得试剂情况选择使用。
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| 关 键 词： | 伤寒 免疫诊断 抗原 抗体 血清 |
The Status on Immunodiagnostic Methods of Typhoid Fever |
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| Bao Xinghao,Sanitary and Antiepidemic Center of Zhejiang Province,Hangzhou.The Status on Immunodiagnostic Methods of Typhoid Fever[J].Bulletin of Science and Technology,1991,7(6):354-359. |
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| Authors: | Bao Xinghao Sanitary and Antiepidemic Center of Zhejiang Province Hangzhou |
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| Abstract: | In this paper, the immunodiagnostic methods of typhoid fever are evalu- ated with author's own research work. Immunodiagnostic methods of typhoid fever could be divided into two groups: (1) Detecting antigen. The SPA-CoA and ELISA (including Dot- ELISA) are sensitive and specific, but DRLAT is simpler and quicker; (2) Detecting antibody. The PHA is the simplest and quickest method. The LPS- PHA is used for rapid diagnosis of typhoid fever of acute-phase, and Vi-PHA may be used to screen the carriers of post-typhoid fever. ELISA is higher sensitive than PHA. The LPS-IgM-ELISA can be used as early diagnosis of typhoid fever however; LPS-IgG-ELISA can be trace back diagnosis and seroepidemiological surveillance in the post-typhoid fever disease. Vi-ELISA may also be applied for screening carriers in the post-typhoid fever patients. Different Laboratories can select different agents according to their own conditions and acquired reagents. |
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| Keywords: | typhoid fever immunodiagnostic methods antigen antibody passive hemoagglutination (PHA) enzyme-linked-immunosorbent assay (ELISA) virulence lipopolysaccharide ( LPS ) |
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