Rapid,one-step fabrication and loading of nanoscale 1,2-distearoyl-sn-glycero-3-phosphocholine liposomes in a simple,double flow-focusing microfluidic device |
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| Authors: | Ryan V Tien Sing Young Maryam Tabrizian |
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| Institution: | 1Department of Biomedical Engineering, McGill University, Duff Medical Building, Montreal, Quebec H3A 2B4, Canada;2Faculty of Dentistry, McGill University, Strathcona Anatomy & Dentistry Building, Montreal, Quebec H3A 2B2, Canada |
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| Abstract: | Liposomes are currently well-established as biocompatible delivery vehicles for numerous compounds. However, conventional manufacturing tends to rely on time-consuming processes, costly equipment, unstable reaction parameters, and numerous pre- and post-processing steps. Herein, we demonstrate a microscope-slide-sized alternative: a double flow-focusing microfluidic geometry capable of sub-hour synthesis and controlled loading of tunable liposomes. Using phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine as the bilayer constituent, the effect of varying the dissolved lipid concentration and flow rate ratio on synthesized liposome diameters was investigated and the encapsulation of fluorescent hydrophobic drug model ergost-5,7,9(11),22-tetraen-3β-ol was performed to ascertain the potential of this device as a loading platform. |
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