| 8-羟基喹啉与鲱鱼精DNA作用方式的光谱法研究 |
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| 引用本文： | 杨欢,王兴明.8-羟基喹啉与鲱鱼精DNA作用方式的光谱法研究[J].商丘师范学院学报,2012,28(9):49-52. |
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| 作者姓名： | 杨欢 王兴明 |
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| 作者单位： | 西南科技大学,材料科学与工程学院化学系,四川绵阳621010 |
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| 基金项目： | 国家自然科学基金资助项目(30973634) |
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| 摘 要： | 在pH=7.40的Tris-HCl缓冲溶液中,采用荧光光谱法和粘度法研究了8-羟基喹啉（HQ）与鲱鱼精DNA的作用方式.用摩尔比法确定了HQ与DNA的结合比为12∶1.通过热力学研究得出,在27℃时HQ与DNA之间相互作用的Kφ27℃=3.844×105L/mol,热力学函数ΔrHφm=-5.08×104J/mol,ΔrGφm 300.15K=-3.2×104J/mol,ΔrSφm=-62.6 J/（mol.K）.结果显示该反应为焓驱动.实验表明HQ与DNA的作用方式为部分嵌插与静电作用方式.
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| 关 键 词： | 鲱鱼精DNA 8-羟基喹啉 溴化乙锭 荧光光谱 作用方式 |
Study on the interaction mode between 8-Hydroxyquiuoline and DNA by spectrometry |
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| YANG Huan,WANG Xingming.Study on the interaction mode between 8-Hydroxyquiuoline and DNA by spectrometry[J].Journal of Shangqiu Teachers College,2012,28(9):49-52. |
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| Authors: | YANG Huan WANG Xingming |
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| Institution: | (Department of Chemistry,School Materials Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China) |
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| Abstract: | The mechanism of interaction between HQ and DNA has been studies by fluorescence spectra and viscosity measurements in solution of Tris-HCl(pH=7.4).From mole ration method,the mole ration between HQ and DNA is 12∶1.Through the study of the thermodynamic we known that binding constant of HQ interaction with DNA is Kφ25℃=3.844×105 L/mol,the corresponding thermodynamic parameters are ΔrHφm=–5.08×104 J/mol,ΔrGφm300.15K=-3.2×104 J/mol,ΔrSφm=-62.6 J/(mol·K).The result suggested that enthalpy was the driven force in this reaction.And the interaction of HQ and DNA through by electrostatic interaction and partial intercalation method. |
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| Keywords: | herring sperm DNA 8-Hydroxyquiuoline ethidium bromide fluorescence spectra interaction method |
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