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Alteration of certain soil microbiological and biochemical indices of a paddy soil under anthropogenic stress
Authors:Abid Subhani  Liao Min  Huang Chang-Yong  Xie Zheng-miao
Institution:(1) College of Environmental and Resource Sciences, Department of Resources Science, Zhejiang University, 310029 Hangzhou, China
Abstract:A 21-day laboratory incubation experiment was conducted to investigate the impact of pesticides (insecticide, herbicide, fungicide) on paddy field soil health under controlled moisture (flooded soil) and temperature (25°C) environment. The electron transport system (ETS)/Dehydrogenase activity showed negative correlation with pesticides concentrations, decreased with increase of pesticide concentration. The higher doses (5 to 10 times field rates) of pesticides significantly inhibited ETS activity, while lower rates failed to produce any significant reducing effect on the control. The toxicity of pesticides in decreasing the ETS activity was in the order: insecticide > fungicide > herbicide, irrespective of their rates of application. The pesticides increased the soil phenol content, which increased with increasing concentration of agrochemicals. The pesticide application did not produce any significant change in soil protein content. The response of biomass phospholipid content was nearly similar to that of ETS activity. The phospholipid content decreased with the addition of pesticides in the order insecticide > fungicide > herbicide and the toxicity was in the order: 10 FR (field rate) > 5 FR > 1.0 FR > 0.5 FR > control. Project (No. G1999011809) supported by the National Key Basic Research Foundation (973) of China Ph. D. student at Zhejiang University, from Ministry of Agriculture (Punjab), Government of Pakistan
Keywords:Electron transport system (ETS) activity  Phenol  Phospholipids  Protein  Pesticides  Submerged soil
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