Preliminary studies on the antitubercular activity and the mechanism of action of the water extract of garlic (Allium sativum) and its two partially purified proteins (Garlic defensins?) |
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| Authors: | P Ratnakar P Suryanarayana Murthy |
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| Institution: | 1. Department of Biochemistry, University College of Medical Sciences and G.T.B. Hospital, Shahdara, 110 095, Delhi
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| Abstract: | Water extract of garlic (Allium sativum) inhibited the growth ofMycobacterium tuberculosis H37Rv andM. tuberculosis TRC-C1193 susceptible and resistant to isoniazid respectively. The minimum inhibitory concentration (MIC) was slightly above 80 but less than 160 μg/ml and slightly above 100 but less than 200 μg/ml for the susceptible and resistant strains respectively. Gel filtration in Sephadex G-100 columns showed that two protein fractions (43 & 38 kD) possessed antitubercular activity with much lower MICs of 20–40 μg/ml and 30–60 μg/ml for susceptible strain. Water extract, when added to actively growingM. tuberculosis in their mid log phase prevented their further growth. The water extract of garlic inhibited the incorporation of14C glycine into whole cells by 81% in 6 hrs. indicating that the primary mechanism of action is by inhibition of protein synthesis. |
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