| 耗竭运动对大鼠肝脏线粒体能量转换功能的影响 |
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| 引用本文： | 时庆德,张勇,陈家琦,刘树森.耗竭运动对大鼠肝脏线粒体能量转换功能的影响[J].天津体育学院学报,1997,12(2):20-23. |
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| 作者姓名： | 时庆德 张勇 陈家琦 刘树森 |
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| 作者单位： | 天津体育学院运动医学研究所 |
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| 基金项目： | 天津市教委重点学科课题 |
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| 摘 要： | 以ＳＤ大鼠递增负荷跑台跑为运动模型，观察了耗竭运动对大鼠肝脏线粒体能量转换功能的影响。应用极谱法分别测定了以苹果酸＋谷氨酸为呼吸底物和琥珀酸为呼吸底物时线粒体态４呼吸速率（Ｒ４）、态３呼吸速率（Ｒ３）、呼吸控制比（ＲＣＲ）、及磷氧比（ＡＤＰ／Ｏ）。结果显示，苹果酸＋谷氨酸为呼吸底物时，Ｒ４增加６４．７６％（Ｐ＜０．００１），ＲＣＲ下降１５．０５％（Ｐ＜０．００１），ＡＤＰ／Ｏ下降１２．１７％（Ｐ＜０．０１）。以琥珀酸为底物时，Ｒ４增力０２３．６７％（Ｐ＜０．０５），ＲＣＲ下降１２．３１％（Ｐ＜０．０５），ＡＤＰ／Ｏ下降１１．７６％（Ｐ＜０．０１）。表明高能质子非特异性渗漏增加，氧化磷酸化偶联程度降低。以苹果酸＋谷氨酸为呼吸底物的Ｒ３显著性增高４１．１４％（Ｐ＜０．００１），推测是由体内ＡＴＰ水平下降，致使线粒体呼吸速率代偿性加快。以琥珀酸为底物的Ｒ３略有增高，但没有显著差异。实验结果表明，耗竭运动后肝线粒体苹果酸＋谷氨酸启动的呼吸及琥珀酸启动的呼吸都受到损害。而且，前者损害程度大于后者。
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| 关 键 词： | 耗竭运动，肝脏线粒体，能量转换，大鼠 |
EFFECTS OF EXHAUSTIVE EXERCISE ON ENERGY TRANSFORMATION OF RAT LIVER MITOCHONDRIA |
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| Shi Qingde,Zhang Yong,Chen Jiaqi and Liu Shusen.EFFECTS OF EXHAUSTIVE EXERCISE ON ENERGY TRANSFORMATION OF RAT LIVER MITOCHONDRIA[J].Journal of Tianjin Institute of Physical Education,1997,12(2):20-23. |
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| Authors: | Shi Qingde Zhang Yong Chen Jiaqi and Liu Shusen |
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| Institution: | Shi Qingde Zhang Yong Chen Jiaqi Liu Shusen |
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| Abstract: | The present study observes effects of exhaustive exercise on the energy transformation of rat liver mitochondria using treadmill run of SD rats with incremental load as the exercise model. The state 4 respiration rate (R4) , state 3 respiration rate ( R3 ) , respiration control ratio (RCR) and phosphorus/oxygen (ADP/O ) were deter- mined in the presence of malate and glutamate (M G) or succinate (S) by polarogram method. Results show that t R4 increases by 64. 76 % (P < 0. 001 ) , RCR decreases by 15. 05% (P<0. 001 ) and ADP/O decreases by 12. 17% (P<0. 01 %) respectively in the presence of M G. R4 increases by 23. 67 % (P< 0. 05 ) , RCR decreases by 1 2. 31% (P<0. 05) and ADP/O decreases by 11. 76% (P<0. 01 ) respectively in the pres- ence of S. Results indicate that the high energy proton leak increases and degree of ox- idative phosphorylation coupling decreases. R3 increases significantly by 41. 14 % (P< 0. 001 ) in the presence of M G and it is speculated that the increase of R3 is due to the compensatory increase of respiration rate in mitochondria resulting from the de- crease of ATP. R3 increases a little without significance in the presence of S. This article suggests that the liver mitochondrial respiration induced by M G or S is dam- aged after exhaustive exercise and damage of the former is more severe than that of the latter. |
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| Keywords: | exhaustive exercise liver mitochondria energy transformation rat |
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