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短程硝化-ANAMMOX反应器处理城市污水过程中脱氮微生物的群落解析
作者姓名:王惠  易鹏  张树军  刘研萍  陶莹  周军  刘新春
作者单位:1. 中国科学院研究生院资源与环境学院, 北京 100049; 2. 北京城市排水集团有限责任公司, 北京 100022; 3. 北京化工大学环境科学与工程系, 北京 100029; 4. 河南源通环保工程有限公司, 河南开封 475000
基金项目:国家科技支撑计划(2007BAD87B12)和中国科学院知识创新工程重要方向项目基金(KSCX2-YW-G-054)资助
摘    要:对基于短程硝化-厌氧氨氧化反应体系实现低氨氮城市生活污水的稳定脱氮进行了实验分析. 稳定时,反应体系的总氮去除率达80%. 对反应器活性污泥中脱氮微生物进行了多样性分析. 结果表明,低溶氧的短程硝化反应器中,与Nitrosomonas europaea/Nitrosococcus mobilis相似性较高的氨氧化细菌,以及分别与Nitrobacter vulgaris 和Nitrospira defluvii相似性较高的亚硝酸盐氧化菌是优势菌种;厌氧氨氧化反应器中,与Kuenenia stuttgartiensis相似性较高的厌氧氨氧化细菌是优势菌种. 并对厌氧氨氧化反应器中总细菌的群落结构进行了解析,发现大量属于Proteobacteria门、Chloroflexi门和Bacteroidetes/Chlorobi门的细菌与anammox细菌共存.

关 键 词:短程硝化  厌氧氨氧化  氨氧化细菌  亚硝酸盐氧化菌  
收稿时间:2011-04-12
修稿时间:2011-04-20

Functional microbial community composition analysis in the laboratory-scale stable partial nitrifying-ANAMMOX municipal wastewater reactor
Authors:WANG Hui  YI Peng  ZHANG Shu-Jun  LIU Yan-Ping  TAO Ying  ZHOU Jun  LIU Xin-Chun
Institution:1. College of Resources and Environment, Graduate University of Chinese Academy of Sciences, Beijing 100049, China; 2. Beijing Drainage Group Co Ltd, Beijing 100022, China; 3. Department of Environmental Sciences and Engineering, Beijing University of Chemical Technology, Beijing 100029, China; 4. Yuan Tong Environmental Engineering Co Ltd, Kaifeng 475000, Henan, China
Abstract:A partial nitrification-ANAMMOX reactor system for domestic wastewater was set up, and the composition of N-related functional bacteria in the activated sludge were studied. During the stable period, the TN removal rate reached to about 80%. In the partial nitrification system, the dominant AOB is Nitrosomonas europaea/Nitrosococcus mobilis cluster-like bacteria, and the dominant NOB are Nitrobacter vulgaris-like bacteria and Nitrospira defluvii-like bacteria. In the anammox reactor, Kuenenia stuttgartiensis-like bacteria is the dominant anammox bacteria. Numerous members of proteobacteria, Chloroflexi, and Bacteroidetes/Chlorobi phylum are found to co-exist with anammox bacteria.
Keywords:partial nitrification                                                                                                                        anammox                                                                                                                        ammonium oxidation bacteria (AOB)                                                                                                                        nitrite oxidation bacteria (NOB)
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