| 通过对免疫共沉淀技术的优化验证蛋白质弱相互作用 |
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| 作者姓名： | 冯晓琴 徐峰 王嵬 刘利新 |
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| 作者单位： | 中国科学院研究生院, 北京 100049 |
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| 基金项目： | 国家自然科学基金(30670889,30771193)资助 |
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| 摘 要： | 采用单因子法对影响免疫共沉淀结果的各因素进行优化.以hCLP46(human CAP10-like protein46)蛋白和内质网分子伴侣calnexin为例,对相互作用开展研究.通过对细胞裂解液各组分浓度、抗体用量、hCLP46的蛋白量和交联剂DSP因素的优化,验证了hCLP46(human CAP10-like protein46)蛋白和内质网分子伴侣calnexin间的弱相互作用.研究结果为探讨蛋白质之间弱相互作用提供一定的参考价值.
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| 关 键 词： | 免疫共沉淀 hCLP46 calnexin 影响因素 蛋白质相互作用 |
| 收稿时间： | 2012-02-23 |
| 修稿时间： | 2012-03-15 |
Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation |
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| Authors: | FENG Xiao-Qin XU Feng WANG Wei LIU Li-Xin |
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| Institution: | Graduate University, Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Co-immunoprecipitation is widely used to detect protein-protein interaction in physiological condition. The single-factor method was used to optimize some factors of co-immunoprecipitation, including component concentrations of cell-lysis buffer, antibody dosage, hCLP46 protein quantity, and cross-linking DSP. As a result, the weak interaction between hCLP46 (human CAP10-like protein 46) and ER chaperone calnexin is confirmed. The present work provides an important basis for further study of the protein-protein interaction. |
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| Keywords: | co-immunoprecipitation(Co-IP) hCLP46 calnexin optimization factors protein-protein interaction |
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