| 喜旱莲子草花序正反向SSH cDNA文库的构建及EST分析 |
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| 作者姓名： | 王兰英 陈洁 刘卫 |
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| 作者单位： | 1. 中国科学院华南植物园, 广州 510650;2. 中国科学院研究生院, 北京 100049 |
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| 基金项目： | 国家重点基础研究发展规划(973)项目(2009CB119204)和国家自然科学基金(30530160)资助 |
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| 摘 要： | 利用SSH技术构建了喜旱莲子草雌雄同花和雄蕊心皮化花序的正反向基因表达的cDNA文库. 对文库部分克隆分析发现,EST功能涉及花性别发育、细胞代谢、生长发育、抗性适应、转录调控、酶调节、叶绿体和胞质基因、细胞内组件等8种不同功能类型.共筛选到8类与已知调控花性别发育相关的EST,如HD基因家族、MADS-box基因家族、线粒体基因、CCLS4家族蛋白等.
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| 关 键 词： | 喜旱莲子草 雄蕊心皮化 SSH文库 EST分析 |
| 收稿时间： | 2011-09-13 |
| 修稿时间： | 2011-12-23 |
Construction and EST analysis of suppression subtractive hybridization library of Alternanthera philoxeroides inflorescence |
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| Authors: | WANG Lan-Ying CHEN Jie LIU Wei |
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| Institution: | 1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;2. Graduate University, Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Functions of the SSH cDNA library of two different inflorescences of hermaphrodite and pistillate of Alternanthera philoxeroides were sorted into eight different categories, involving the development of sex differentiation of flowers, the process of metabolism, cytoplasmic gene etc. Sex differentiation ESTs point to eight kinds of gene families, including homeobox ZIP, MADS-box, CCLS4 protein family, mitochondria male-fertile, and cytoplasmic male-sterility genes. |
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| Keywords: | Alternanthera philoxeroides" target="_blank">Alternanthera philoxeroides')">Alternanthera philoxeroides stamen-pistillody SSH library EST analysis |
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