A microfluidic platform for real-time and in situ monitoring of virus infection process |
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| Authors: | Na Xu Zhen-Feng Zhang Li Wang Bo Gao Dai-Wen Pang Han-Zhong Wang Zhi-Ling Zhang |
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| Institution: | 1.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, People’s Republic of China;2.Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, People’s Republic of China;3.State Key Laboratory of Virology, Wuhan, People’s Republic of China |
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| Abstract: | Microfluidic chip is a promising platform for studying virus behaviors at the cell level. However, only a few chip-based studies on virus infection have been reported. Here, a three-layer microfluidic chip with low shear stress was designed to monitor the infection process of a recombinant Pseudorabies virus (GFP-PrV) in real time and in situ, which could express green fluorescent protein during the genome replication. The infection and proliferation characteristics of GFP-PrV were measured by monitoring the fluorescence intensity of GFP and determining the one-step growth curve. It was found that the infection behaviors of GFP-PrV in the host cells could hardly be influenced by the microenvironment in the microfluidic chip. Furthermore, the results of drug inhibition assays on the microfluidic chip with a tree-like concentration gradient generator showed that one of the infection pathways of GFP-PrV in the host cells was microtubule-dependent. This work established a promising microfluidic platform for the research on virus infection. |
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