| 超声波提取—高效液相色谱法测定茶叶中的槲皮素 |
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| 引用本文： | 许志刚,陈云丽,张仙丽,李娟,张燕菊.超声波提取—高效液相色谱法测定茶叶中的槲皮素[J].昆明师范高等专科学校学报,2013(3):66-68. |
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| 作者姓名： | 许志刚 陈云丽 张仙丽 李娟 张燕菊 |
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| 作者单位： | 昆明理工大学理学院,云南昆明650093 |
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| 基金项目： | 国家自然科学基金资助项目（21205054）;云南省应用基础研究资助项目（2011FZ043）;昆明理工大学分析测试基金资助项目（20130568,20130564,20130562） |
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| 摘 要： | 采用超声波提取茶叶中的槲皮素,优化了提取溶剂的体积分数、提取时间和固液比,并联用高效液相色谱法测定4种茶叶中槲皮素的质量分数,色谱条件为V（乙腈）∶V（0.2％磷酸溶液）=30∶70,流速1.0 mL/min,检测波长360 nm,柱温30℃.测定结果表明：普洱熟茶中槲皮素质量分数最高,为0.143 8 mg/g,普洱生茶次之,两种绿茶的质量分数最低,方法精密度在2.5％～4.7％之间.
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| 关 键 词： | 槲皮素 茶叶 超声波提取 高效液相色谱法 |
Determination of Quercetin in Tea by Ultrasonic Extraction Coupled with High Performance Liquid Chromatography |
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| XU Zhi-gang,CHEN Yun-li,ZHANG Xian-li,LI. Juan,ZHANG Yan-ju.Determination of Quercetin in Tea by Ultrasonic Extraction Coupled with High Performance Liquid Chromatography[J].Journal of Kunming Teachers College,2013(3):66-68. |
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| Authors: | XU Zhi-gang CHEN Yun-li ZHANG Xian-li LI Juan ZHANG Yan-ju |
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| Institution: | ( Faculty of Science, Kunming University of Science and Technology, Yunnan Kunming 650093, China) |
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| Abstract: | The quercetin if four teas was determined by ultrasonic extraction coupled with high performance liquid chromatography. The extraction conditions were optimized,including extraction solvent, extraction time and solid-liquid ration. Coupled with high performance liquid chromatography the mass fraction of quercetin in four sorts of tea is determined. The mobile phase was acetonitrile and 0. 2% phosphoric acid solution （the volume ration of 30：70） with a flow rate of 1.0 mL/min. The detection wavelength was 360 nm and the column temperature was 30 ~C. The results showed that the content of quercetion in Puer ripe tea was the highest. It was 0. 143 8 mg/L. The content of quercetion in two green teas was the lowest. The content of quercetion in Puer raw tea was in the middle. The detection precision was in the range of 2. 5% to 4.7%. |
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| Keywords: | quercetin tea ultrasonic extraction high performance liquid chromatography |
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