| 鸡传染性贫血病弱毒苗免疫对肝线粒体抗氧化酶活性的影响 |
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| 引用本文： | 孙素玲,周建.鸡传染性贫血病弱毒苗免疫对肝线粒体抗氧化酶活性的影响[J].安徽科技学院学报,2011,25(5):1-4. |
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| 作者姓名： | 孙素玲 周建 |
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| 作者单位： | 安徽科技学院动物科学学院,安徽凤阳,233100;安徽省饲料工业协会,安徽合肥,230001 |
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| 基金项目： | 安徽省教育厅重点项目(KJ2008A32ZC) |
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| 摘 要： | 观察鸡传染性贫血病弱毒苗多次免疫对肝线粒体中抗氧化酶活性的影响。方法：20只SPF鸡随机分为2组，每组10只，免疫组鸡用鸡传染性贫血弱毒苗免疫四次，每次间隔2W，对照组鸡用同剂量的生理盐水。最后一次免疫后10d取肝脏制备线粒体，利用测试盒测定肝线粒体中的GSH-PX活性、SOD活性和MDA含量。结果：对照组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为20．444±2．64U／mgprot、15．59±1．33U／mgprot和7．32＋1．89nmol／mL，免疫组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为24．85±3．53U／mgprot、24．284±4．17U／mgprot和5．264±1．24nmol／mL。与对照组相比，免疫组肝线粒体中GSH-PX活性显著提高（P〈0．05），SOD活性极显著提高（P〈0．01），MDA含量显著减低（P〈0．05）。结论：鸡传染性贫血病多次免疫可提高机体抗氧化能力。
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| 关 键 词： | 鸡传染性贫血 免疫 肝线粒体 抗氧化物酶活性 |
Effect of Chicken Infectious Anemia Immunity on Antioxidant Enzyme Activity in Liver Mitochondria of Chickens |
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| SUN Su-ling,ZHOU Jian.Effect of Chicken Infectious Anemia Immunity on Antioxidant Enzyme Activity in Liver Mitochondria of Chickens[J].Journal of Anhui Science and Technology University,2011,25(5):1-4. |
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| Authors: | SUN Su-ling ZHOU Jian |
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| Institution: | SUN Su-ling1,ZHOU Jian2(1.College of Animal Saience,Anhui Scinece and Technology University,Fengyang 233100,China,2.Feed Industry Association of Anhui Province,Hefei 230001,China) |
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| Abstract: | Observed the effect of chicken infectious anemia(CIA) immunity on the antioxidant enzyme activity in liver mitochondria of chickens.Method:20 chickens were randomly divided into two groups.There was 10 chickens every group.The chickens in immuned group were immuned by CIA attenuated vaccine 4 times,each time interval of 2 w.The chickens in control group were injected by NS.The liver mitochondria were prepared 10 d after the last immunization.The GSH-PX activity,the SOD activity and the MDA contention in liv... |
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| Keywords: | Chicken infectious anemia Immune Liver mitochondria Antioxidant enzyme activity |
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