| 减量训练通过不同亚型的PI3K对心肌的影响机制 |
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| 引用本文： | 张军.减量训练通过不同亚型的PI3K对心肌的影响机制[J].成都体育学院学报,2016(5):107-112. |
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| 作者姓名： | 张军 |
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| 作者单位： | 燕山大学,河北 秦皇岛,066004 |
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| 基金项目： | 燕山大学博士基金项目“大运动量和减量训练对心肌mTOR信号通路的影响”(B920)；燕山大学青年教师自主研究计划课题“运动训练和减量训练对心肌的影响机制”(15SKB009)。 |
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| 摘 要： | 目的：探讨在减量训练过程中，不同亚型的 PI3K 影响心肌的机制。方法：72只雄性 SD大鼠随机分为：3周安静对照组（C3）和3周大运动量训练组（E3）；3天对照组（C4）和3天减量训练组（E4）；6天对照组（C5）和6天减量训练组（E5）；2周对照组（C6）和2周减量训练组（ E6）。3周大运动量训练和2周的减量训练后取左心室肌组织，测定 PI3K p85、PI3K p110α和PI3K p110γ蛋白采用 Western blotting 方法。心肌组织形态学采用 HE 染色在光镜下观察。结果：3周大运动量训练和2周减量训练后，心肌组织形态没有显著变化，未发现心肌组织损伤。6天减量训练后，心肌 PI3K p110α蛋白表达显著增加（P ＜0.05）。2周减量训练后，心肌 PI3K p110α／ PI3K p85显著增加（P ＜0.05）。3周大运动量训练和减量训练后，心肌 PI3K p110γ蛋白表达没有显著变化（P ＞0.05）。结论：本研究制定的运动训练和减量训练方案未造成心肌损伤。减量训练通过不同亚型的PI3K影响心肌。
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| 关 键 词： | 减量训练 心肌 PI3K p85 PI3K p110α PI3K p110γ |
The Mechanism of Tapering Influencing Cardiac Muscles Through PI3K of Different Subtypes |
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| Abstract: | Objective:To explore the cardiac muscle be affected by PI3K in the course of tapering and its mechanism. Methods:72 male SD rats were randomly divided into 8 groups after one week of adaptability training:the group that rest for 3 weeks (C3),the group that receive large amount of training for 3 weeks (E3);the group that rest for 3 days (C4)and the group that receive 3 days of tapering (E4);the group that rest for 6 days (C5)and the group that receive 6 days of tapering (E5);the group that rest for 2 weeks (C6)and the group that receive 2 weeks of tapering (E6). After 3 weeks of large amount of training and 2 weeks of tapering,the cardiac mus-cle of left ventricle was sampled. PI3K p85,PI3K p110α and PI3K p110γ protein was tested through Western blotting. The morphological structure of cardiac muscle organization was HE colored and observed under light microscope. Results:After 3 weeks of large amount of training and 2 weeks of tapering,no significant change occurred in the conformation of cardiac muscle organizations. No injuries were observed in the cardiac muscles. PI3K p110α protein expression increased significantly in cardiac muscles after 6 days of tapering (P < 0. 05). PI3K p110γ didn’t change significantly after 3 weeks of large amount of training and after tapering (P >0. 05). Conclusions:The training and tapering in the project didn’t result in any injuries of the cardiac mus-cles. Cardiac muscles can be affected by tapering through PI3K. |
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| Keywords: | Tapering Cardiac Muscle PI3K p85 PI3K p110α PI3K p110γ |
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