| 全脑缺血再灌注大鼠海马Caspase-3、8表达及HWTX-I神经保护机制的研究 |
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| 引用本文： | 刘仁仪,陈嘉勤,毛海峰,王一蓉.全脑缺血再灌注大鼠海马Caspase-3、8表达及HWTX-I神经保护机制的研究[J].北京体育大学学报,2008,31(10). |
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| 作者姓名： | 刘仁仪 陈嘉勤 毛海峰 王一蓉 |
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| 作者单位： | 湖南师范大学体育学院，湘南学院公共课部，宜春学院体育学院，湖南体育职业学院 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 为观察虎纹捕鸟蜘蛛毒素-I(HWTX-I)对全脑缺血再灌注大鼠海马组织凋亡相关因子Caspase-3、Cas-pase-8mRNA及蛋白表达的影响。将SD大鼠随机分为假手术组、生理盐水组及用药组;采用改良的Pulsinelli"四血管阻断"法制备全脑缺血损伤模型并结合蛛网膜下腔置管术;免疫组织化学法检测Caspase-3、Caspase-8蛋白水平的表达;RT-PCR法检测凋亡相关因子Caspase-3、Caspase-8mRNA表达变化。免疫组织化学法检测结果显示,HWTX-I能显著降低全脑缺血再灌注大鼠海马组织CA1区Caspase-3、Caspase-8蛋白水平的表达(P<0.05);RT-PCR结果显示,HWTX-I能显著降低全脑缺血及再灌注大鼠海马组织Caspase-3、Caspase-8核酸水平的表达(P<0.05)。提示:HWTX-I蛛网膜下腔用药对全脑缺血再灌注SD大鼠海马组织的保护作用可能通过抑制凋亡相关因子Caspase-3、Caspase-8mRNA和蛋白表达,从而发挥其抗脑缺血再灌注损伤的保护作用。
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| 关 键 词： | 虎纹蜘蛛毒素-I 脑缺血再灌注 海马 半胱天冬酶-3 半胱天冬酶-8 |
The Expression of Caspase-3,8 in the Hippocampus of Rat in Global Cerebral Ischemia and Reperfusion and the Mechanism of Neural Protection with HWTX-I |
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| LIU Ren-yi,CHEN Jia-qin,MAO Hai-feng,WANG Yi-rong.The Expression of Caspase-3,8 in the Hippocampus of Rat in Global Cerebral Ischemia and Reperfusion and the Mechanism of Neural Protection with HWTX-I[J].Journal of Beijing Sport University,2008,31(10). |
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| Authors: | LIU Ren-yi CHEN Jia-qin MAO Hai-feng WANG Yi-rong |
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| Institution: | LIU Ren-yi1,2,CHEN Jia-qin1,MAO Hai-feng3,WANG Yi-rong4,1 |
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| Abstract: | To investigate the effects of Huwentoxin-I(HWTX-I) on expression of mRNA and protein of apoptotic correlation factors,such as Caspase-3,Caspase-8 in hippocampus tissue,after global cerebral ischimia and reperfusion in rats.48 female SD rats randomly are divided into the sham-operation group,the physiological saline group and administration group,and each group consisted of 18 rats.the improved global cerebral ischemia damage models of Pulsinelli 4-blood vessal occlusion combined with the method of inserting anaesthesic tube into intrathecal space are adopted to detect the changes of the expression of Caspase-3 and Caspase-8,in protein by immunohistochemistry,and mRNA by RT-PCR.Immunohistochemical staining indicates that HWTX-I can lighten strong positive expression of Caspase-8 and Caspase-3 in hippocampal CA1 region,after global cerebral ischimia and reperfusion in rats(P<0.05),and the expression of Caspase-8 and Caspase-3mRNA in the hippocampus tissue of administration group(P<0.05). It can be concluded that,injected into intrathecal space,HWTX-I tends to have anti-apoptotic role in the hippocampus tissues of rats subjected to transient ischemia,through inhibiting the expression of mRNA and protein of apoptotic correlation factors Caspase-3 and Caspase-8. |
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| Keywords: | HWTX-I cerebral ischemia and reperfusion hippocampus Caspase-3(p20) Caspase-8(p10) |
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