| HPLC法测定广玉兰中小白菊内酯 |
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| 引用本文： | 丁林芬,郭亚东,吴兴德,秦智彬,张菊,马银海.HPLC法测定广玉兰中小白菊内酯[J].昆明师范高等专科学校学报,2012(6):79-80. |
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| 作者姓名： | 丁林芬 郭亚东 吴兴德 秦智彬 张菊 马银海 |
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| 作者单位： | [1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204 [3]云南中医学院药学院,云南昆明650500 [4]昆明学院化学科学与技术系,云南昆明650214 |
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| 摘 要： | 建立高效液相色谱法测定广玉兰中小白菊内酯含量的方法．采用ZORBAXSB—C18（4．6mm×150mm，5μm）色谱柱，以甲醇（A）-水（B）为流动相，梯度洗脱（0～10min，10％—48％A；10～20min，48％-80％A），流速1．0mL／min，柱温30℃，检测波长210nm．结果表明，小白菊内酯的线性范围为0．188～2．820μg（r=0．9999），平均回收率（n=9）为99．3％．证实该方法简便、准确、重复性好，可用于广玉兰中小白菊内酯的含量测定．
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| 关 键 词： | 高效液相色谱法 广玉兰 小白菊内酯 含量测定 |
Determination of Parthenolide in Magnolia Grandiflora L. by the Method of HPLC |
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| DING Lin-fen,GUO Ya-dong,WU Xing-de,QIN Zhi-bin,ZHANG Ju,MA Yin-hai.Determination of Parthenolide in Magnolia Grandiflora L. by the Method of HPLC[J].Journal of Kunming Teachers College,2012(6):79-80. |
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| Authors: | DING Lin-fen GUO Ya-dong WU Xing-de QIN Zhi-bin ZHANG Ju MA Yin-hai |
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| Institution: | 1. College of Pharmaceutical Science ＆ Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Yunnan Kunming 650500, China;2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Kunming 650204, China ;3. College of Pharmacy, Yunnan University of Traditional Chinese Medicine, Yunnan Kunming 650500, China ;4. Department of Chemistry Science and Technology, Kunming University, Yunnan Kunming 650214, China) |
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| Abstract: | To develop a HPLC method for determination of Parthenolide in Magnolia Grandiflora L. , a ZORBAX SB - C18 （4. 6 mm x 150 mm, 5 μm） column was adopted. The mobile phase consists of methanol（A） -water（B） for gradient elution （0-10 min, 10%-48% A; 10-20 min, 48%-80% A） , which was pumped at 1.0 mL/min. The detection wavelength was at 210 nm, and the column temperature was maintained at 30 ℃. The results suggested that the linear ranges was 0. 188-2. 820μg （r =0. 999 9） and the average recovery （n = 9） was 99. 3%. The method is simple, rapid, reliable, and can be used for the determination of Magnolia Grandlora L.. |
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| Keywords: | HPLC Magnolia Grandiflora L parthenolide content determination |
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