Protective Role of Catechin on <Emphasis Type="SmallCaps">d</Emphasis>-Galactosamine Induced Hepatotoxicity Through a p53 Dependent Pathway |
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| Authors: | P Vasanth Raj K Nitesh S Sagar Gang V Hitesh Jagani H Raghu Chandrashekhar J Venkata Rao C Mallikarjuna Rao N Udupa |
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| Institution: | (1) Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104, Karnataka, India;(2) Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104, Karnataka, India; |
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| Abstract: | Objective of this study was to obtain a better understanding of the mechanism responsible for the d-galactosamine (d-GalN) induced hepatotoxicity and to study the effect of catechin against d-GalN induced hepatotoxicity. Catechin 50 and 100 mg/kg b.wt was administered for 1 week by oral route. Liver damage was induced
by intra-peritoneal administration of 400 mg/kg b.wt d-galactosamine on the last day of catechin treatment. At the end of treatment all animals were killed and liver enzyme levels
were estimated. Dissected hepatic samples were used for histopathology, RNA isolation, expression studies of Bax, Bcl-2 and
p53 mRNA levels and mitochondrial membrane potential studies. We found that increases in the liver enzyme activity and decrease
in antioxidant enzyme activity by d-GalN were significantly restricted by oral pretreatment with catechin. Disruption of mitochondrial membrane potential, up
regulation of p53, Bax and down regulation of Bcl-2 mRNA levels in the liver of d-GalN intoxicated rats were effectively prevented by pretreatment with catechin. |
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| Keywords: | Mitochondria Catechin d-Galactosamine p53 Bax Bcl-2 Hepatoprotective |
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