| 胃癌ZAC基因的杂合性缺失检测 |
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| 引用本文： | 张建勋,朱运良.胃癌ZAC基因的杂合性缺失检测[J].常熟理工学院学报,2011,25(4):71-74. |
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| 作者姓名： | 张建勋 朱运良 |
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| 作者单位： | 郑州大学基础医学院法医教研室,郑州,450001 |
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| 摘 要： | 以30例胃癌组织和正常组织为研究对象,通过STR-PCR技术,对6号染色体6q24内的ZAC基因两侧的4个STR基因座进行杂合性缺失分析,统计这4个STR基因座在30例标本中的LOH频率,绘制它们的缺失图谱,得到了第4号和25号这两个样本中发生了包含ZAC基因在内的4285651bp的缺失,为进一步分析ZAC基因发生缺失的断裂点和查找ZAC基因表达下调的原因打下基础.
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| 关 键 词： | 杂合性缺失 STR 6号染色体 ZAC基因 |
The Loss of Heterozygosity of the ZAC Gene Detected in Gastric Tumor |
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| ZHANG Jian-xun,ZHU Yun-liang.The Loss of Heterozygosity of the ZAC Gene Detected in Gastric Tumor[J].Journal of Changshu Institute of Technology,2011,25(4):71-74. |
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| Authors: | ZHANG Jian-xun ZHU Yun-liang |
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| Institution: | ZHANG Jian-xun,ZHU Yun-liang(Department of Forensic Medicine,Medical College,Zhengzhou University,Zhengzhou 450001,China) |
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| Abstract: | To investigate the loss of heterozygosity(LOH) of four STR loci of near ZAC gene on the long arm of 6 chromosome(6q24.2),STR-PCR method was used to detect four loci LOH in gastric tumor and paired normal control tissues of thirty cases.Four STR loci was counted for LOH frequency in the 30 cases,mapping their deletion map,getting 4285651bp deletion including ZAC gene in fourth and fifth samples.It laid a foundation both for further analysis of ZAC gene deletion breaking point and finding the reasons for ZAC ... |
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| Keywords: | loss of heterozygosity(LOH) Short tandem repeat(STR) 6q ZAC gene |
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