| 一种纳豆芽孢杆菌的分子鉴定及纳豆激酶基因克隆 |
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| 引用本文： | 李建强,石洪凌,肖冬光.一种纳豆芽孢杆菌的分子鉴定及纳豆激酶基因克隆[J].唐山师范学院学报,2013(2):37-40. |
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| 作者姓名： | 李建强 石洪凌 肖冬光 |
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| 作者单位： | 天津科技大学生物工程学院;唐山师范学院生命科学系 |
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| 摘 要： | 以实验室保藏的一株具有溶解血栓能力的芽孢杆菌（LJQ—NK）为出发菌株，通过形态学分析和16SrRNA比对，对菌株进行鉴定，更进一步利用聚合酶链式反应（PCR）克隆纳豆激酶DNA片段⑤16SrRNA比对。结果表明LJQ-NK与已报道的纳豆激酶16SrRNA序列有99．9％以上的同源性，初步认定为一株纳豆芽孢杆菌。克隆获得1120bp的DNA片段，应用DNAMAN软件分析，与已报道的纳豆激酶编码基因（G129164926）存在两个碱基的差异，编码纳豆激酶开放阅读框区域序列完全相同。
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| 关 键 词： | 纳豆芽孢杆菌 纳豆激酶 16S rRNA比对 |
Molecular Indentification of Bacillus Subtilis Natto and Clone Gene ofNattokinase |
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| LI Jian-qiang,SHI Hong-ling,XIAO Dong-guang.Molecular Indentification of Bacillus Subtilis Natto and Clone Gene ofNattokinase[J].Journal of Tangshan Teachers College,2013(2):37-40. |
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| Authors: | LI Jian-qiang SHI Hong-ling XIAO Dong-guang |
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| Institution: | 1(1.College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300000,China;2.Department of Life Science,Tangshan Teachers College,Tangshan 063000,China) |
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| Abstract: | LJQ-NK, a strain with ability of fibrinolysis was identified by molecular biology method. Sequence analysis shows that the homology of 16S rRNA between LJQ-NK and Bacillus subtilis natto was exceeded 99. 9%. The putative nattokinase cDNA was cloned from LJQ-NK using RT-PCR and a 1 126 bp cDNA clone was obtained. Result of BLAST-X sequence homology search show that two bases of putative nattokinase cDNA clone were differed from nattokinase and the ORF sequence ofnattokinase between LJQ-NK and Bacillus subtilis natto were identical. |
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| Keywords: | Bacillus subtilis natto Nattokinase 16S rRNA gene alignment |
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